Enhanced Proliferation of Porcine Bone Marrow Mesenchymal Stem Cells Induced by Extracellular Calcium is Associated with the Activation of the Calcium-Sensing Receptor and ERK Signaling Pathway

文献类型： 外文期刊

第一作者： Ye, Jingjing

作者： Ye, Jingjing;Ai, Wei;Zhang, Fenglin;Zhu, Xiaotong;Shu, Gang;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, YongLiang;Jiang, Qingyan;Wang, Songbo;Ye, Jingjing;Ai, Wei;Zhang, Fenglin;Zhu, Xiaotong;Shu, Gang;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, YongLiang;Jiang, Qingyan;Wang, Songbo;Ye, Jingjing;Ai, Wei;Zhang, Fenglin;Zhu, Xiaotong;Shu, Gang;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, YongLiang;Jiang, Qingyan;Wang, Songbo;Ye, Jingjing;Ai, Wei;Zhang, Fenglin;Zhu, Xiaotong;Shu, Gang;Wang, Lina;Gao, Ping;Xi, Qianyun;Zhang, YongLiang;Jiang, Qingyan;Wang, Songbo

作者机构：

期刊名称：STEM CELLS INTERNATIONAL （ 影响因子：5.443； 五年影响因子：5.305 ）

ISSN： 1687-966X

年卷期： 2016 年

页码：

收录情况： SCI

摘要： Porcine bone marrow mesenchymal stem cells (pBMSCs) have the potential for application in regenerative medicine. This study aims to investigate the effects of extracellular calcium ([Ca2+](o)) on pBMSCs proliferation and to explore the possible underlying mechanisms. The results demonstrated that 4mM [Ca2+](o) significantly promoted pBMSCs proliferation by reducing the G0/G1 phase cell percentage and by increasing the S phase cell proportion and the proliferation index of pBMSCs. Accordingly, [Ca2+](o) stimulated the expression levels of proliferative genes such as cyclin A2, cyclin D1/3, cyclin E2, and PCNA and inhibited the expression of p21. In addition, [Ca2+](o) resulted in a significant elevation of intracellular calcium and an increased ratio of pERK/ERK. However, inhibition of calcium-sensing receptor (CaSR) by its antagonist NPS2143 abolished the aforementioned effects of [Ca2+](o). Moreover, [Ca2+](o)-induced promotion of pBMSCs proliferation, the changes of proliferative genes expression levels, and the activation of ERK1/2 signaling pathway were effectively blocked by U0126, a selective ERK kinase inhibitor. In conclusion, our findings provided evidence that the enhanced pBMSCs proliferation in response to [Ca2+](o) was associated with the activation of CaSR and ERK1/2 signaling pathway, which may be useful for the application of pBMSCs in future clinical studies aimed at tissue regeneration and repair.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序