The flavohemoglobin gene MoFHB1 is involved in the endurance against nitrosative stress in Magnaporthe oryzae

文献类型： 外文期刊

第一作者： Zhang, Zhen

作者： Zhang, Zhen;Hao, Zhongna;Chai, Rongyao;Qiu, Haiping;Wang, Jiaoyu;Wang, Yanli;Sun, Guochang

作者机构：

关键词： Magnaporthe oryzae; flavohemoglobin; NO degradation

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.82； 五年影响因子：3.248 ）

ISSN： 0378-1097

年卷期： 2022 年 369 卷 1 期

页码：

收录情况： SCI

摘要： Nitric oxide (NO) homeostasis plays a versatile role in pathogen-host interactions. To maintain NO homeostasis in favor of pathogens, microbes have evolved NO degradation systems besides NO synthesis pathway, in which the flavohemoglobin and S-nitrosoglutathione (GSNO) reductase are two key enzymes. We previously proved that MoSFA1, a GSNO reductase, is required for the growth and pathogenicity in Magnaporthe oryzae. In the present work, MoFHB1, a flavohemoglobin-encoding gene in M. oryzae was functionally characterized. Although the expression of the MoFHB1 gene was developmentally regulated during conidial germination and appressorium development, disruption of MoFHB1 did not change vegetative growth, conidiation and virulence. However, compared with the Delta mosfa1 mutant, the Delta mofhb1 mutant was significantly more sensitive to NO stress, and the expression of MoSFA1 gene in the Delta mofhb1 mutant was significantly upregulated. Double deletion of MoSFA1 and MoFHB1 led to greater sensitivity of the fungus to NO stress than either of the single gene mutant, but no further reduction in pathogenicity was found compared with that of Delta mosfa1 mutant. Taken together, MoFHB1 played an important role in NO detoxification but was dispensable for virulence of M. oryzae. The flavohemoglobin MoFHB1 of Magnaporthe oryzae is involved in the endurance against nitrosative stress and not necessary for pathogenicity.

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