Characterization of MicroRNA* Species in Peking Duck Skin

文献类型： 外文期刊

第一作者： Zhang Li

作者： Zhang Li;Xiao Mei;An Li-long;Xie Xiu-juan;Lin Shu-dai;Luo Wen;Jia Xin-zheng;Nie Qing-hua;Zhang Xi-quan;Jia Shan-gang

作者机构：

关键词： Peking duck;miRNA star species (miRNA*);skin;nucleotide bias

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2013 年 12 卷 9 期

页码：

收录情况： SCI

摘要： A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3'-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the "seed region". miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.

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