Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

文献类型： 外文期刊

第一作者： Gan, J. K.

作者： Gan, J. K.;Zhang, D. X.;Zhang, X. Q.;Chen, Z. Y.;Luo, Q. B.;Gan, J. K.;Zhang, D. X.;He, D. L.;Zhang, X. Q.;Chen, Z. Y.;Luo, Q. B.;He, D. L.

作者机构：

关键词： HSP70;DNA methylation;Heat inducible

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2013 年 12 卷 1 期

页码：

收录情况： SCI

摘要： DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dropping. Hypomethylation of the HSP70 promoter occurred in all of the groups studied, but the difference between groups within tissue type was not significant. The DNA methylation level of the control and the 6-h treatment groups was slightly higher than that of the 3-h treatment group in brain tissue and leg muscle. Correlation analysis between mRNA expression and DNA methylation of HSP70 showed that DNA methylation was negatively associated with mRNA expression in leg muscle (P = 0.0124), indicating that DNA methylation may be negatively associated with the expression of HSP70, although the difference was not significant. We concluded that the expression of HSP70 is heat inducible and tissue dependent and that heat induction may correlate with DNA methylation pattern in the HSP70 promoter, whereas tissue dependence is unrelated to DNA methylation pattern.

分类号：

  • 相关文献

[1]Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Xu, Yanhao,Zhong, Lan,Wang, Jianbo,Wu, Xiaoming,Fang, Xiaoping.

[2]Accumulation and subcellular localization of heat shock proteins in young grape leaves during cross-adaptation to temperature stresses. Zhang, Jun-Huan,Pan, Qiu-Hong,Zhan, Ji-Cheng,Huang, Wei-Dong,Zhang, Jun-Huan,Wang, Yu-Zhu,Wang, Li-Jun. 2008

[3]Effect of High Dietary Carbohydrate on Growth, Serum Physiological Response, and Hepatic Heat Shock Protein 70 Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures. Zhou, Chuanpeng,Liu, Bo,Xie, Jun,Ge, Xianping,Liu, Bo,Xie, Jun,Ge, Xianping. 2013

[4]Analysis of genetic diversity of the heat shock protein 70 gene on the basis of abundant sequence polymorphisms in chicken breeds. Gan, J. K.,Kong, L. N.,Zhang, X. Q.,Luo, Q. B.,Gan, J. K.,Gan, J. K.,Jiang, L. Y.,Kong, L. N.,Zhang, X. Q.,Luo, Q. B.. 2015

[5]Molecular cloning and expression of two HSP70 genes in the Wuchang bream (Megalobrama amblycephala Yih). Ming, Jianhua,Xie, Jun,Xu, Pao,Ge, Xianping,Liu, Bo,He, Yijin,Cheng, Yanfen,Zhou, Qunlan,Pan, Liangkun,Ming, Jianhua,Liu, Wenbin. 2010

[6]The Effect of Muscle-Regulatory Factor Genes and Satellite Cell Response to Recombinant Hsp70 Protein on Megalobrama amblycephala Skeletal Muscle. Zhu, Kecheng,Zhang, Dianchang,Wang, Huanling,Zhu, Kecheng,Zhang, Dianchang,Wang, Huanling. 2016

[7]Starvation reduces the heat shock protein responses in white sturgeon larvae. Huang, Susie S. Y.,Hung, Silas S. O.,Han, Dong,Wang, Wei-Fang. 2012

[8]Differential gene expression in whitefly (Bemisia tabaci) B-biotype females and males under heat-shock condition. Wan, Fang-Hao,Wan, Fang-Hao.

[9]Large-scale mortality and limited expression of heat shock proteins of aestivating sea cucumbers Apostichopus japonicus after acute salinity decrease. Meng, Xian-Liang,Dong, Yun-Wei,Meng, Xian-Liang,Dong, Shuang-Lin.

[10]Biochemical and genetic toxicity of dinotefuran on earthworms (Eisenia fetida). Liu, Tong,Wang, Xiuguo,Xu, Jinli,You, Xiangwei,Chen, Dan,Wang, Fenglong,Li, Yiqiang.

[11]Heat shock protein 70 is necessary for Rice stripe virus infection in plants. Jiang, Shanshan,Li, Kunfeng,Lin, Lin,Zheng, Hongying,Chen, Jianping,Jiang, Shanshan,Lu, Yuwen,Li, Kunfeng,Yan, Fei,Chen, Jianping.

[12]Roles of heat-shock protein 70 in protecting against intestinal mucosal damage. Wu, Xin,Zhang, Yuzhe,Yin, Yulong,Wu, Xin,Yin, Yulong,Ruan, Zheng,Wu, Xin,Yin, Yulong,Ruan, Zheng,Yu, Huimin,Wu, Zhenlong,Wu, Guoyao,Wu, Guoyao. 2013

[13]Comparative toxicity and bioaccumulation of two dinotefuran metabolites, UF and DN, in earthworms (Eisenia fetida). Liu, Tong,Zhang, Xiaolian,Wang, Xiuguo,Chen, Dan,Li, Yiqiang,Wang, Fenglong. 2018

[14]Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology. Huang Zhihui,Huang Zhihui,Ma Aijun,Wang Xin'an,Lei Jilin,Li Weiye,Wang Ting,Yang Zhi,Qu Jiangbo. 2015

[15]Physiological responses and HSP70 mRNA expression in GIFT tilapia juveniles, Oreochromis niloticus under short-term crowding. Jun, Qiang,Hong, Yang,Jie, He,Pao, Xu,Jun, Qiang,Hong, Yang,Didlyn, Kpundeh Mathew,Pao, Xu,Hui, Wang.

[16]Effects of anthraquinones extracted from Rheum officinale Bail on the growth, non-specific immune response of Macrobrachium rosenbergii. Liu, Bo,Ge, Xianping,Xie, Jun,Xu, Pao,Liu, Bo,Ge, Xianping,He, Yanhui,Xie, Jun,Xu, Pao,He, Yijin,Zhou, Qunlan,Pan, Liangkun,Chen, Ruli. 2010

[17]Lipopolysaccharide and heat stress impair the estradiol biosynthesis in granulosa cells via increase of HSP70 and inhibition of smad3 phosphorylation and nuclear translocation. Li, Hui,Cai, Liuping,Shi, Zhendan,Guo, Shuangshuang,Ma, Weiming.

[18]Molecular characteristics of the HSP70 gene and its differential expression in female and male golden apple snails (Pomacea canaliculata) under temperature stimulation. Song, Hong-Mei,Mu, Xi-Dong,Gu, Dang-En,Luo, Du,Yang, Ye-Xin,Xu, Meng,Luo, Jian-Ren,Hu, Yin-Chang,Zhang, Jia-En.

[19]Repeated handling compromises the immune suppression and improves the disease resistance in overwintering channel catfish (Ictalurus punctatus). Yang, Bingyuan,Hu, Huihua,Tu, Yongqin,Han, Dong,Zhu, Xiaoming,Jin, Junyan,Yang, Yunxia,Xie, Shouqi,Yang, Bingyuan,Hu, Huihua,Tu, Yongqin,Wang, Cuicui,Han, Dong.

[20]Effects of temperature and dietary protein on gene expression of Hsp70 and Wap65 and immunity of juvenile mirror carp (Cyprinus carpio). Huang, Jin-feng,Xu, Qi-you,Chang, Yu-mei,Huang, Jin-feng.

作者其他论文 更多>>

微信小程序