The second transcribed spacer rDNA sequence: an effective genetic marker for inter similar to species phylogenetic analysis of trematodes in the order Strigeata
文献类型： 外文期刊
第一作者： Li, J.
作者： Li, J.;Mo, X. H.;Li, X. Y.;Song, H. Q.;Zhu, X. Q.;Zhao, G. H.;Li, X. Y.;Li, J.;Li, J.;Lin, R. Q.;Weng, Y. B.;Song, H. Q.;Zou, F. C.;Zhu, X. Q.
作者机构：
期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）
ISSN： 0932-0113
年卷期： 2012 年 111 卷 4 期
页码：
收录情况： SCI
摘要： In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA of Schistosoma japonicum isolates in mainland China was amplified, sequenced, and assessed for inferring the intra-and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment containing ITS-2 rDNA was obtained from 24 S. japonicum isolates from eight epidemic provinces in mainland China. The length polymorphisms were observed among these ITS-2 rDNA sequences, ranging from 343 to 346 bp, and the intra-and inter-population variations in ITS-2 sequence were 0.0-2.1% among S. japonicum isolates in China. Phylogenetic analyses using the maximum parsimony and maximum likelihood methods revealed that the ITS2 rDNA sequence is not a suitable marker for studying inter- and intra-population variation in S. japonicum. However, phylogenetic analysis of trematodes in the order Strigeata indicated that the ITS-2 rDNA sequence provides an effective molecular marker for studying inter-species phylogenetic relationships among trematodes in this order.
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