Biochemical Characterization of the Outer Membrane Enzyme OMPLA from Riemerella anatipestifer

文献类型: 外文期刊

第一作者: Liao, Shenquan

作者: Liao, Shenquan;Yuan, Jianfeng;Qin, Zonghua;Wu, Caiyan;Lv, Minna;Qi, Nanshan;Cai, Jianping;Sun, Mingfei

作者机构:

关键词: Riemerella anatipestifer;outer membrane phospholipase A;methyl arachidonyl fluorophosphonate;protein;database

期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )

ISSN: 1680-5593

年卷期: 2012 年 11 卷 21 期

页码:

收录情况: SCI

摘要: A gene (pld4) encoding a 33.0 kDa protein. with significant homology to the Escherichia coli outer membrane phospholipase A was identified from Riemerella anatipestifer genome database. The results of this study revealed that this gene encoded an Outer Membrane Phospholipase A (OMPLA) in Riemerella anatipestifer. The recombinant ONEPLA protein. was expressed and its general biochemical features were also, determined. Methyl Arachidonyl Fluorophosphonate (MAFP) could specifically inhibit the OMPLA activity. These observations suggest that OMPLA could be exploited as a drug target in the bacteria and MAFP may serve as a lead compound for drug discovery against R. anatipestifer.

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