Biochemical Characterization of the Outer Membrane Enzyme OMPLA from Riemerella anatipestifer
文献类型: 外文期刊
第一作者: Liao, Shenquan
作者: Liao, Shenquan;Yuan, Jianfeng;Qin, Zonghua;Wu, Caiyan;Lv, Minna;Qi, Nanshan;Cai, Jianping;Sun, Mingfei
作者机构:
关键词: Riemerella anatipestifer;outer membrane phospholipase A;methyl arachidonyl fluorophosphonate;protein;database
期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )
ISSN: 1680-5593
年卷期: 2012 年 11 卷 21 期
页码:
收录情况: SCI
摘要: A gene (pld4) encoding a 33.0 kDa protein. with significant homology to the Escherichia coli outer membrane phospholipase A was identified from Riemerella anatipestifer genome database. The results of this study revealed that this gene encoded an Outer Membrane Phospholipase A (OMPLA) in Riemerella anatipestifer. The recombinant ONEPLA protein. was expressed and its general biochemical features were also, determined. Methyl Arachidonyl Fluorophosphonate (MAFP) could specifically inhibit the OMPLA activity. These observations suggest that OMPLA could be exploited as a drug target in the bacteria and MAFP may serve as a lead compound for drug discovery against R. anatipestifer.
分类号:
- 相关文献
作者其他论文 更多>>
-
Polymerization of beneficial plant height QTLs to develop superior lines which can achieving hybrid performance levels
作者:Kang, Congbin;Hao, Yichen;Sun, Mingfei;Li, Mengyao;Tian, Ziang;Zhao, Yajie;Zhou, Chao;Zhao, Xiang Yu;Zhang, Xian Sheng;Yang, Xuerong;Liu, Hongjun;Zhang, Lin;Dong, Ling;Liu, Xianjun;Zeng, Xing;Sun, Yanjie;Cao, Shiliang;Luebberstedt, Thomas
关键词:Heterosis; Complex agronomic traits; Plant height; Molecular design; Pyramiding QTL line (PQL); Maize
-
Structure-Based Virtual Screening of Potential Inhibitors Targeting the Prolyl-tRNA Synthetase (PRS) in Eimeria tenella: Insights from Molecular Docking, ADMET Studies, and Molecular Dynamics Simulations
作者:Cai, Haiming;Liao, Shenquan;Li, Juan;Lv, Minna;Lin, Xuhui;Song, Yongle;Chen, Xiangjie;Zhu, Yibin;Zhang, Jianfei;Qi, Nanshan;Sun, Mingfei
关键词:avian coccidiosis; prolyl-tRNA synthetase;
Eimeria tenella ; docking protocol; molecular dynamics simulations; ADMET -
Development of a single-cell cloning technique for isolation of Pentatrichomonas hominis: a promising tool for diagnosing Trichomonas spp. infections in the pig breeding industry
作者:Zhu, Yibin;Cai, Haiming;Liao, Shenquan;Li, Juan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Chen, Xiangjie;Yin, Lijun;Zhang, Jianfei;Qi, Nanshan;Sun, Mingfei;Fang, Siyun;Wang, Dingai;Yan, Zhuanqiang;Shen, Hanqin
关键词:
Trichomonas spp.; Isolation method; Single-cell cloning -
Research note: The critical role of the interaction between Eimeria tenella invasion protein RON2 and host receptor annexin A2 in mediating parasite invasion
作者:Ni, Junli;Chen, Xiangjie;Sun, Yunyun;Zhu, Yibin;Cai, Haiming;Song, Yongle;Lv, Minna;Li, Juan;Liao, Shenquan;Qi, Nanshan;Sun, Mingfei;Zhang, Jianfei;Lin, Xuhui;Ni, Junli;Sun, Yunyun;Gu, Youfang;Liu, Xinchao
关键词:Avian coccidia; Eimeria tenella; RON2; Invasion; Protein; receptor interactions
-
Prevalence and genetic diversity of Pentatrichomonas hominis in pig populations in Guangdong and Anhui Provinces, China
作者:Lu, Pengyun;Zhu, Yibin;Cai, Haiming;Liao, Shenquan;Qi, Nanshan;Lv, Minna;Lin, Xuhui;Song, Yongle;Chen, Xiangjie;Zhang, Jianfei;Li, Juan;Sun, Mingfei;Lu, Pengyun;Shen, Hanqin;Fang, Siyun;Wang, Dingai;Yan, Zhuanqiang
关键词:
Pentatrichomonas hominis ; Pig infection; Zoonotic potential; Prevalence; Genetic diversity -
Unraveling the pathogenic potential of the Pentatrichomonas hominis PHGD strain: impact on IPEC-J2 cell growth, adhesion, and gene expression
作者:Zhu, Yibin;Cai, Haiming;Qi, Nanshan;Li, Juan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Chen, Xiangjie;Yin, Lijun;Zhang, Jianfei;Liao, Shenquan;Sun, Mingfei;Fang, Siyun;Yan, Zhuanqiang;Wang, Dingai;Shen, Hanqin
关键词:Pentatrichomonas hominis; IPEC-J2; cell viability; inflammatory response
-
Metabolic Blockade-Based Genome Mining of Sea Anemone-Associated Streptomyces sp. S1502 Identifies Atypical Angucyclines WS-5995 A-E: Isolation, Identification, Biosynthetic Investigation, and Bioactivities
作者:Wang, Yuyang;Zhou, Le;Ju, Jianhua;Ma, Junying;Wang, Yuyang;Ju, Jianhua;Ma, Junying;Pan, Xiaoting;Qi, Nanshan;Sun, Mingfei;Liao, Zhangjun;Zhang, Hua
关键词:metabolic blockade-based genome mining; Streptomyces sp. S1502; WS-5995; biosynthesis; anticoccidial activity