A Rapid and Cost Effective Method for Genotyping Two Types of GHR Mutations in Sex-Linked Dwarf Chicken

文献类型： 外文期刊

第一作者： Shu, Ding-Ming

作者： Shu, Ding-Ming;Wang, Yan;Qu, Hao;Luo, Cheng-Long;Wang, Jie;Shu, Ding-Ming

作者机构：

关键词： GHR;sex-linked dwarf chicken;genotyping;mutation;stable

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 11 期

页码：

收录情况： SCI

摘要： Large deletion or mutation at the exon 10 and 3' untranslated region of Growth Factor Receptor (GHR) gene results in dwarf chicken and the common method for detecting mutation is direct PCR amplification. But since, the target fragment is large, the amplification is inefficient and unreliable and this process consumes a lot of expensive specific DNA polymerases. Researchers focused on the point mutation C146280A in the deletion fragment and set up a new method to detect large deletion and point mutation in this region. Comparing with direct PCR this new method which can detect both large deletion and point mutation with regular low-cost DNA polymerases is more stable, reliably and cheapness.

分类号：

  • 相关文献

[1]Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation. Tian, Jian,Wang, Ping,Gao, Shan,Chu, Xiaoyu,Wu, Ningfeng,Fan, Yunliu.

[2]Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.). Liu, Qing-Long,Xu, Xiu-Hong,Ren, Xue-Liang,Fu, Hao-Wei,Wu, Dian-Xing,Shu, Qing-Yao.

[3]Leptin stimulates hepatic activation of thyroid hormones and promotes early posthatch growth in the chicken. Li, Rongjie,Hu, Yan,Ni, Yingdong,Zhao, Ruqian,Li, Rongjie,Xia, Dong,Hu, Yan,Grossmann, Roland.

[4]The Effects of Different Sex-Linked Dwarf Variations on Chinese Native Chickens. Ouyang Jian-hua,Xie Liang,Nie Qing-hua,Zeng Hua,Zhang De-xiang,Zhang Xi-quan,Ouyang Jian-hua,Ouyang Jian-hua,Peng Zhi-jun,Zhang De-xiang,Xie Liang. 2012

[5]Associations between GHR and IGF-1 gene polymorphisms, and reproductive traits in Wenchang chickens. Li, Hui-fang,Zhu, Wen-qi,Chen, Kuan-wei,Tang, Qing-ping,Gao, Yu-shi,Wu, Xu. 2008

[6]Comparative Study on the Genetic Diversity of GHR Gene in Tibetan Cattle and Holstein Cows. Deng, Feilong,Jia, Xianbo,Lai, Song-Jia,Chen, Shi-Yi,Xia, Chenyang,Song, Tianzeng,Liu, Jianzhi.

[7]Castration-induced changes in the expression profiles and promoter methylation of the GHR gene in Huainan male pigs. Wang, Jing,Chen, Junfeng,Zhang, Jiaqing,Gao, Binwen,Bai, Xianxiao,Lan, Yali,Lin, Ping,Guo, Hongxia,Xing, Baosong,Gao, Yuan.

[8]Development of a core set of SNP markers for the identification of upland cotton cultivars in China. Kuang Meng,WEI Shou-jun,WANG Yan-qin,ZHOU Da-yun,MA Lei,FANG Dan,YANG Wei-hua,MA Zhi-ying. 2016

[9]Primary surveys on molecular epidemiology of bovine viral diarrhea virus 1 infecting goats in Jiangsu province, China. Mao, Li,Li, Wenliang,Yang, Leilei,Zhang, Wenwen,Hao, Fei,Jiang, Jieyuan,Wang, Jianhui,Wei, Yong,Cheng, Suping,Wang, Qiusheng,Ding, Yonglong,Sun, Yinhua. 2016

[10]Association Analysis in Rice: From Application to Utilization. Zhang, Peng,Zhong, Kaizhen,Tong, Hanhua,Shahid, Muhammad Qasim. 2016

[11]Ovine prion protein genotype frequencies in northwestern China. Zhao, C. -L.,Wu, R.,Liu, L.,Zhang, X. -L.,Wang, C.,Wang, F.,Diao, X. -L.,Guan, H. -W.,Wang, X.,Zhou, L.,Wu, R.,Li, F. -D.. 2012

[12]New Genotypes of Enterocytozoon bieneusi Isolated from Sika Deer and Red Deer in China. Huang, Jianying,Zhang, Zhenjie,Wang, Rongjun,Zhao, Jinfeng,Jian, Fuchun,Ning, Changshen,Zhang, Longxian,Yang, Yong,Yang, Yong. 2017

[13]Diversity maintenance and use of Vicia faba L. genetic resources. Duc, Gerard,Bao, Shiying,Baum, Michael,Redden, Bob,Sadiki, Mohammed,Jose Suso, Maria,Vishniakova, Margarita,Zong, Xuxiao. 2010

[14]Characterization of Novel Di-, Tri-, and Tetranucleotide Microsatellite Primers Suitable for Genotyping Various Plant Pathogenic Fungi with Special Emphasis on Fusaria and Mycospherella graminicola. Abd-Elsalam, Kamel A.,Bahkali, Ali H.,Abd-Elsalam, Kamel A.,Verreet, Joseph-Alexander,Abd-Elsalam, Kamel A.,Khiyami, Mohamed A.,Guo, Jian-Rong. 2012

[15]Allelic variation and geographic distribution of vernalization genes HvVRN1 and HvVRN2 in Chinese barley germplasm. Dondup, Dawa,Xu, Dongdong,Zhang, Lisha,Zha, Sang,Yuan, Xingmiao,Zhang, Jing,Guo, Ganggang,Dondup, Dawa,Tashi, Nyima,Dong, Guoqing,Zhang, Lisha.

[16]Identification and pathotypical analysis of a novel VIk sub-genotype Newcastle disease virus obtained from pigeon in China. Xue, Cong,Xu, Xiaohong,Yin, Renfu,Qian, Jing,Cong, Yanlong,Ding, Zhuang,Xue, Cong,Xu, Xiaohong,Yin, Renfu,Qian, Jing,Cong, Yanlong,Ding, Zhuang,Sun, Yixue,Wang, Chunfeng,Ding, Chan,Yu, Shengqing,Hu, Shunlin,Liu, Xiufan.

[17]Genetic diversity of the genotype VII Newcastle disease virus: identification of a novel VIIj sub-genotype. Xue, Cong,Cong, Yanlong,Yin, Renfu,Qian, Jing,Yuan, Qianliang,Yang, Mingxi,Ding, Zhuang,Xue, Cong,Cong, Yanlong,Yin, Renfu,Qian, Jing,Yuan, Qianliang,Yang, Mingxi,Ding, Zhuang,Sun, Yixue,Wang, Chunfeng,Ding, Chan,Yu, Shengqing,Liu, Xiufan,Hu, Shunlin,Wang, Chunfeng.

[18]Enhancing genetic gain in the era of molecular breeding. Xu, Yunbi,Zou, Cheng,Xie, Chuanxiao,Xu, Yunbi,Zhang, Xuecai,Li, Ping,Lu, Yanli,Prasanna, Boddupalli M.,Olsen, Michael S..

[19]Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle, northwest China. Zhang, Xiao-Xuan,Tan, Qi-Dong,Zhou, Dong-Hui,Ni, Xiao-Ting,Liu, Guang-Xue,Yang, Yan-Chuan,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Tan, Qi-Dong.

[20]Prevalence, risk factors and molecular characterization of Enterocytozoon bieneusi in raccoon dogs (Nyctereutes procyonoides) in five provinces of Northern China. Xu, Chao,Zhang, Xiao-Xuan,Rui-Du,Wang, Quan-Kai,Zhao, Quan,Xu, Chao,Zhang, Hao,Zhao, Jia-Ping,Ba, Heng-Xing,Xing, Xiu Mei,Ma, Xusheng. 2016

作者其他论文 更多>>

微信小程序