The anti-inflammatory effect of the SOCC blocker SK&F 96365 on mouse lymphocytes after stimulation by Con A or PMA/ionomycin

文献类型： 外文期刊

第一作者： Ye, Yanxia

作者： Ye, Yanxia;Zhang, Yaxing;Lu, Xiaoyu;Huang, Xiuyan;Zeng, Xiangfeng;Zeng, Yaoying;Lu, Xiaoyu;Lai, Xinqiang

作者机构：

关键词： SK&F 96365;Lymphocytes;Con A;PMA/ionomycin;Proliferation;MPT;Anti-inflammatory

期刊名称：IMMUNOBIOLOGY （ 影响因子：3.144； 五年影响因子：3.18 ）

ISSN： 0171-2985

年卷期： 2011 年 216 卷 9 期

页码：

收录情况： SCI

摘要： SK&F 96365, 51-(beta-(3-(p-methoxyphenyl)-propyloxyl-p-methoxyPhenethyl)-1H-imidazole hydrochloride, has emerged as a useful pharmacological tool in the study of store-operated Ca2(+) entry (SOCE). But the precise molecular mechanism and effect of SK&F 96365 on mouse lymphocytes are still not well determined. This study investigated the pharmacological profile of SK&F 96365 on mouse lymphocytes stimulated by mitogen concanavalin A (Con A) or by a combination of a protein kinase C (PKC) activator, phorbol 12-myristate 13-acetate (PMA) and a calcium ionophore, ionomycin in vitro. Our results showed that SK&F 96365 pre-treatment diminished the cytosolic calcium rise on lymphocytes induced by ionomycin, PMA/ionomycin, and thapsigargin (TG), respectively. CFDA-SE staining results showed that SK&F 96365 (5-20 mu M) inhibited both Con A- and PMA/ionomycin-induced lymphocytes proliferation in a time- and dose-dependent manner. Upon the same stimulation, SK&F 96365 inhibited the expression of CD69 and CD25 on CD3(+) T lymphocytes in a dose-dependent manner. The cell cycle analyzing results showed that SK&F 96365 caused a G0/G1 phase cell cycle arrest on both Con A- and PMA/ionomycin-activated lymphocytes in a dose-dependent manner. In addition, SK&F 96365 induced a decrease in mitochondrial membrane potential (Delta Psi m) and promoted mitochondrial permeability transition (MPT) in both Con A- and PMA/ionomycin-activated lymphocytes. Furthermore, SK&F 96365 significantly inhibited the production of proinflammatory cytokines (interferon (IFN)-gamma and tumor necrosis factor (TNF)), and the anti-inflammatory cytokine (IL-10) on both Con A- and PMA/ionomycin-activated lymphocytes. SK82F 96365 did not induce a statistically significant increase in levels of proinflammatory IL-6 and monocyte chemoattractant protein-1 (MCP-1) but of IL-12p70 upon the stimulation of Con A, whereas these three cytokines were markedly inhibited by it upon the stimulation of PMA/ionomycin. This finding revealed that SK&F 96365 exhibited an anti-inflammatory effect on mouse lymphocytes both upon the stimulation of Con A and PMA/ionomycin, and the precise mechanism of SK&F 96365 inhibiting Con A-activated lymphocytes proliferation is different from PMA/ionomycin. (C) 2011 Elsevier GmbH. All rights reserved.

分类号：

  • 相关文献

[1]Protective effect of Potentilla anserine polysaccharide (PAP) on hydrogen peroxide induced apoptosis in murine splenic lymphocytes. Hu, TingJun,Wei, Xiaojie,Zhang, Ling,Kang, Le,Zhang, Xia,Shuai, XueHong,Cheng, FuSheng.

[2]Immunotoxicity of aluminum. Zhu, Yanzhu,Miao, Liguang,Wang, Yingping,Liu, Yanhuan,Yan, Xijun,Cui, Xuezhe,Li, Haitao,Zhu, Yanzhu,Li, Yanfei.

[3]Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. Lim, Yourim,Park, Ji-Won,Kwon, Ok-Kyoung,Lee, Jae-Won,Lee, Han-Sol,Ahn, Kyung-Seop,Lim, Yourim,Lee, Han-Sol,Han, Sang-Bae,Lee, Sangwoo,Choi, Sangho,Li, Wanyi,Jin, Hang. 2018

[4]Egg-Derived Peptide IRW Inhibits TNF-alpha-Induced Inflammatory Response and Oxidative Stress in Endothelial Cells. Chakrabarti, Subhadeep,Jiang, Yanyan,Davidge, Sandra T.,Chakrabarti, Subhadeep,Jiang, Yanyan,Davidge, Sandra T.,Chakrabarti, Subhadeep,Jiang, Yanyan,Davidge, Sandra T.,Huang, Wuyang,Majumder, Kaustav,Wu, Jianping,Huang, Wuyang. 2010

[5]Inhibitory Effects of 4 '-Demethylnobiletin, a Metabolite of Nobiletin, on 12-O-Tetradecanoylphorbol-13-acetate (TPA)-Induced Inflammation in Mouse Ears. Wu, Xian,Song, Mingyue,Rakariyatham, Kanyasiri,Zheng, Jinkai,Wang, Minqi,Xu, Fei,Gao, Zili,Xiao, Hang,Xiao, Hang,Zheng, Jinkai.

[6]Lonicera japonica Thunb.: Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. Shang, Xiaofei,Li, Maoxing,Li, Maoxing,Ding, Hong,Shang, Xiaofei,Pan, Hu,Miao, Xiaolou. 2011

[7]Ethnobotany, phytochemistry and pharmacology of the genus Caragana used in traditional Chinese medicine. Meng, Qiuxia,Roubin, Rebecca H.,Hanrahan, Jane R.,Meng, Qiuxia,Niu, Yu,Niu, Xiwu. 2009

[8]UPLC-Q-Exactive Orbitrap/MS-Based Lipidomics Approach To Characterize Lipid Extracts from Bee Pollen and Their in Vitro Anti-Inflammatory Properties. Li, Qiangqiang,Liang, Xinwen,Xue, Xiaofeng,Wang, Kai,We, Liming,Li, Qiangqiang,Liang, Xinwen,Xue, Xiaofeng,Wang, Kai,We, Liming,Li, Qiangqiang,Liang, Xinwen,Xue, Xiaofeng,Wang, Kai,Zhao, Liang,Zhang, Zhongyin.

[9]Identification of bioactives from Astragalus chinensis L.f. and their antioxidant, anti-inflammatory and anti-proliferative effects. Hu, Ting,Hu, Ting,Liu, Qi-Mei,He, Xiao-Wei,Jiang, Jian-Guo,Huang, Fei,Zhang, Ming-Wei.

[10]Molecular cloning, sequence analysis, and function of the intestinal epithelial stem cell marker Bmi1 in pig intestinal epithelial cells. Li, C. -M.,Yan, H. -C.,Fu, H. -L.,Xu, G. -F.,Wang, X. -Q.. 2014

[11]The Influence of Thidiazuron on Proliferation of Acer x freemanii In Vitro. Zhao, X. Q.,Yuan, Z. H.,Yin, Y. L.,Feng, L. J.,Yin, Y. L.,Xu, R.. 2012

[12]Effect of Auxins on In Vitro Propagation of Sour Jujube Infected by Witches' Broom Phytoplasma. Sun, Q.,Zhou, G.,Sun, H.,Giang, G.. 2009

[13]Effects of linoleic acid and eicosapentaenoic acid on cell proliferation and lipid-metabolism gene expression in primary duck hepatocytes. Liu, W. M.,Lu, L. Z.,Zhang, C.,Liu, Y. L.,Zhang, J.,Tao, Z. R.,Shen, J. D.,Li, G. Q.,Wang, D. Q.,Li, J. J.,Tian, Y.,Liu, W. M.,Shi, F. X.,Liu, Y. L.,Zhang, J.. 2011

[14]Up-regulation of miR-182-5p predicts poor prognosis in patients with lung cancer and associates with tumor cell growth and migration. Shi, Yuanyuan,Liu, Hao,Du, Xiang,Liu, Xuedong,Liu, Jing. 2017

[15]Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Sun, Yingning. 2013

[16]Integrated Analyses Reveal Overexpressed Notch1 Promoting Porcine Satellite Cells' Proliferation through Regulating the Cell Cycle. Jiao, Yiren,Huang, Bo,Chen, Yu,Hong, Guangliang,Xu, Jian,Wang, Chong,Hu, Chingyuan. 2018

[17]FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells. Li, Shuying,Jiang, Zhonghao,Xu, Wenyi,Xie, Yingying,Zhao, Leiming,Tang, Xuanming,Wang, Fengzhong,Xin, Fengjiao.

[18]PDPN gene promotes the proliferation of immature Bovine Sertoli cells in vitro. Gao, Yi,Qin, Lihong,Zhang, Guoliang,Gao, Yi,Dong, Xue,Zhao, Zijiao,Yang, Yuwei,Zhao, Zhihui.

[19]Effects of Daidzein or Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow in vitro. Liu, Chunlong,Li, Zhongqiu,Feng, Xingjun. 2012

[20]Characterization of a new fungal immunomodulatory protein, FIP-dsq2 from Dichomitus squalens. Li, Shuying,Jiang, Zhonghao,Sun, Lichao,Liu, Xin,Huang, Ying,Wang, Fengzhong,Xin, Fengjiao.

作者其他论文 更多>>

微信小程序