Proteomic analysis of purified coronavirus infectious bronchitis virus particles

文献类型： 外文期刊

第一作者： Kong, Qingming

作者： Kong, Qingming;Xue, Chunyi;Zhang, Chengwen;Li, Linlin;Cao, Yongchang;Shu, Dingming;Bi, Yingzuo;Ren, Xiangpeng

作者机构：

期刊名称：PROTEOME SCIENCE （ 影响因子：2.48； 五年影响因子：2.619 ）

ISSN： 1477-5956

年卷期： 2010 年 8 卷

页码：

收录情况： SCI

摘要： Background: Infectious bronchitis virus (IBV) is the coronavirus of domestic chickens causing major economic losses to the poultry industry. Because of the complexity of the IBV life cycle and the small number of viral structural proteins, important virus-host relationships likely remain to be discovered. Toward this goal, we performed two-dimensional gel electrophoresis fractionation coupled to mass spectrometry identification approaches to perform a comprehensive proteomic analysis of purified IBV particles. Results: Apart from the virus-encoded structural proteins, we detected 60 host proteins in the purified virions which can be grouped into several functional categories including intracellular trafficking proteins (20%), molecular chaperone (18%), macromolcular biosynthesis proteins (17%), cytoskeletal proteins (15%), signal transport proteins (15%), protein degradation (8%), chromosome associated proteins (2%), ribosomal proteins (2%), and other function proteins (3%). Interestingly, 21 of the total host proteins have not been reported to be present in virions of other virus families, such as major vault protein, TENP protein, ovalbumin, and scavenger receptor protein. Following identification of the host proteins by proteomic methods, the presence of 4 proteins in the purified IBV preparation was verified by western blotting and immunogold labeling detection. Conclusions: The results present the first standard proteomic profile of IBV and may facilitate the understanding of the pathogenic mechanisms.

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