a-Linolenic acid alleviates aluminum toxicity in RAW264.7 cells by antioxidative and anti-inflammatory effects

文献类型： 外文期刊

第一作者： Ji, Qiqi

作者： Ji, Qiqi;Han, Ling;Xia, Xiaoyang;Xiang, Xia;Ji, Qiqi;Zhang, Tianyuan

作者机构：

关键词： a-linolenic acid; Inflammation; Aluminum; RAW264; 7 cells; RNA-seq

期刊名称：ARABIAN JOURNAL OF CHEMISTRY （ 影响因子：6.0； 五年影响因子：5.9 ）

ISSN： 1878-5352

年卷期： 2023 年 16 卷 8 期

页码：

收录情况： SCI

摘要： Aluminum is an inorganic contaminant and has adverse effects on human and animal health by mainly accumulating in the immune system to exert toxicity. a-Linolenic acid (ALA), a crucial dietary component, possesses various bioactivities. Nevertheless, the effects of ALA on Al-induced toxicity have not been sufficiently understood. In this study, we aimed to elucidate the possible mechanism of aluminium chloride hexahydrate (AlCl3.6H2O, Al) exposure to RAW264.7 cells and the response of ALA through an RNA sequencing (RNA-seq). RNA-seq results demonstrated that Al exposure resulted in 2106 differentially expressed genes (DEGs) with 53 pathways, including the NF-jB, enriched by the KEGG analysis. 200 of the 2106 DEGs were modulated in ALA-pretreated group, which were enriched into 36 pathways, containing the NFjB. qRT-PCR results displayed the RNA-seq's reproducibility and reliability. Western blot analysis confirmed that the protection of ALA against Al toxicity was associated with NF-jB mediated JAK2/STAT3/SOCS3 pathway and HO-1 activation. These results elucidated the protection of ALA against Al-induced toxicity from a new perspective and provided a potential therapy for Al

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