Starch processing wastewater as a new medium for production of Bacillus thuringiensis
文献类型: 外文期刊
第一作者: Chang, Ming
作者: Chang, Ming;Lu, Na;Ni, Jin-Ren;Chang, Ming;Lu, Na;Ni, Jin-Ren;Zhou, Shun-Gui
作者机构:
关键词: starch processing wastewater;waste reuse;Bacillus thuringiensis subsp kurstaki;bioinsecticide;fermentation
期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )
ISSN: 0959-3993
年卷期: 2008 年 24 卷 4 期
页码:
收录情况: SCI
摘要: Production of Bacillus thuringiensis (Bt) based bioinsecticide was studied by using starch processing wastewater (SPW) as a raw material. Results indicated that the nutrients contained in SPW were sufficient for growth, sporulation and delta-endotoxin production of Bacillus thuringiensis subsp. kurstaki (Btk). The final cell counts and spore counts achieved in SPW medium were 72% and 107% respectively higher than those in the soybean meal based commercial medium. Higher 6-endotoxin yield of 2.67 mg mL(-1) and higher entomotoxicity of 1,050 IU mu L-1 were also obtained in SPW medium as compared with the commercial medium at the end of fermentation. The morphological observations also revealed that the fermentation cycle of Btk could be shortened in this new medium. This process provides solutions for safe SPW disposal and production of high potency and low cost bioinsecticide.
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