Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China

文献类型: 外文期刊

第一作者: Katiyar, SK

作者: Katiyar, SK;Tan, Y;Huang, B;Chandel, G;Xu, Y;Zhang, Y;Xie, Z;Bennett, J

作者机构:

关键词: rice (Oryza sativa);gall midge;Orseolia oryzae;RAPD;mapping;sequence tagged sites

期刊名称:THEORETICAL AND APPLIED GENETICS ( 影响因子:5.699; 五年影响因子:5.565 )

ISSN: 0040-5752

年卷期: 2001 年 103 卷 6-7 期

页码:

收录情况: SCI

摘要: The Chinese rice cultivar Duokang #1 carries a single dominant gene Gm-6(t) that confers resistance to the four biotypes of Asian rice gall midge (Orseolia oryzae Wood-Mason) known in China. Bulked segregant analysis was performed on progeny of a cross between Duokang #1 and the gall midge-susceptible cultivar Feng Yin Zhan using the RAPD method. The RAPD marker OPM06((1400)) amplified a locus linked to Gm-6(t). The locus was subsequently mapped to rice chromosome 4 in a region flanked by cloned RFLP markers RG214 and RG163. Fine mapping of Gm-6(t) revealed that markers RG214 and RG476 flanked the gene at distances of LO and 2.3 cM, respectively. Another gall midge resistance gene, Gm-2, mapped previously to chromosome 4. is located about 16 cM from Gm-6(t). to judge by data from a segregating population derived from a cross between Duokang #1 and the Indian cultivar Phalguna that carries Gm-2. We developed a PCR-based marker-assisted selection kit for transfer of the Gm-6(t) gene into Ming Hui 63 and IR50404, two parental lines commonly used in hybrid rice production in China. The kit contains PCR primer pairs based on the terminal sequences of the RG214 and RG476 clones. Polymorphism between Duokang #1 and the hybrid parental lines was found at these markers after digestion of the PCR products with specific restriction endonucleases. The kit will accelerate introduction of gall midge resistance into hybrid rice in China.

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