Isolation and identification of an actin gene from Babesia gibsoni

文献类型： 外文期刊

第一作者： Zhou, JL

作者： Zhou, JL;Huang, B;Suzuki, H;Fujisaki, K;Igarashi, I;Xuan, XN

作者机构：

期刊名称：JOURNAL OF PARASITOLOGY （ 影响因子：1.276； 五年影响因子：1.486 ）

ISSN： 0022-3395

年卷期： 2006 年 92 卷 1 期

页码：

收录情况： SCI

摘要： Actin is I ubiquitous and highly conserved microfilament protein that is hypothesized to play a mechanical force-generating role in the unusual gliding motility of sporozoan zoites and their active penetration of host cells. We have identified and isolated an actin gene from a Babesia gibsoni cDNA library by random sequencing. The complete nucleotide sequence of the actin gene is 1,243 bp; a single open reading frame encodes a polypeptide of 377 amino acid residues. The deduced amino acid sequence showed a high homology with actins from other species. especially with reported apicomplexan protozoans. The antiserum against recombinant actin expressed in Escherichia coli recognizes a 42-kDa native protein. which is consistent with its expected size. Immunofluorescence and confocal microscopic observation revealed that the protein is diffusely distributed throughout the B. gibsoni parasites.

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