Identification of a follistatin-related protein Haemaphysalis longicornis and its effect on from the tick tick oviposition

文献类型： 外文期刊

第一作者： Zhou, JL

作者： Zhou, JL;Liao, M;Hatta, T;Tanaka, M;Xuan, XN;Fujisaki, K

作者机构：

关键词： RNA interference;activin;bone morphogenetic protein

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2006 年 372 卷

页码：

收录情况： SCI

摘要： The identification of ovary-associated molecules will lead to a better understanding of the physiology of tick reproduction and vector-pathogen interactions. A gene encoding a follistatin-retated protein (FRP) was obtained by random, sequencing from the ovary cDNA library of the tick Haemaphysalis longicornis. The full-length cDNA is 1157 bp, including an intact ORF encoding an expected protein with 289 amino acids. Three distinct domains were present in the deduced amino acids, namely, the follistatin-like domain, KAZAL, and two calcium-binding motifs, EFh. The sequence shows homology with the follistatin-related protein (FRP), which was thought to play some roles in the negative regulation of cellular growth. RT-PCR showed that the gene was expressed throughout the developing stages and mainly in the ovary as well as in fat body, hemocytes, salivary glands, and midgut. This gene was expressed in GST-fused recombinant protein with an expected size. The mouse antiserum against the recombinant protein recognized a 56-kDa native protein in both tick ovary and hemolymph. The recombinant proteins were found to have binding activity for both activin A and bone morphogenetic protem-2 (BMP-2). Silencing of FRP by RNAi showed a decrease in tick oviposition, which is consistent with the effect of a recombinant protein vaccine on the adult tick. These results showed that the tick FRP might be involved in tick oviposition. This is the first report of a member of follistatin family proteins in Chelicerata, which include ticks, spiders, and scorpions. (c) 2006 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Cloning, expression profiling and promoter functional analysis of bone morphogenetic protein 2 in the tongue sole (Cynoglossus semilaevis). Ma, Qian,Fan, Yanjun,Zhuang, Zhimeng,Liu, Shufang,Ma, Qian,Liu, Shufang,Zhuang, Zhimeng. 2018

[2]PHYSIOLOGICAL AND BIOCHEMICAL PROPERTIES ANALYSIS OF LATE-BOLTING TRANSGENIC CHINESE CABBAGE (BRASSICA RAPA L. SSP PEKINENSIS). Xia, G.,He, Q.,Zhao, S.. 2015

[3]Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference. Ma, Jin,Song, Yunzhi,Wu, Bin,Li, Kaidong,Zhu, Changxiang,Wen, Fujiang,Jiang, Mingsong. 2011

[4]Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Jiang, Yayuan,Sun, Lin,Li, Kaidong,Song, Yunzhi,Zhu, Changxiang,Jiang, Mingsong.

[5]Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus. Dai, Su-Ming,Xiao, Luo,Xie, Bing-Yan,Cheng, Xin-Yue. 2010

[6]Identification of Hop stunt viroid infecting Citrus limon in China using small RNAs deep sequencing approach. Fu, Shuai,Qian, Yajuan,Xu, Yi,Zhou, Xueping,Zhou, Xueping. 2015

[7]Peptidoglycan Recognition Protein S2 From Silkworm Integument: Characterization, Microbe-Induced Expression, and Involvement in the Immune-Deficiency Pathway. Yang, Jie,Wang, Xiaonan,Wu, Jinmei,Tang, Shunming,Shen, Zhongyuan,Wu, Jinmei. 2015

[8]RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus. Fang, Ying,Lee, Seok Hee,Kim, Jong Hoon,Park, Dong Hwan,Park, Min Gu,Woo, Ra Mi,Lee, Bo Ram,Kim, Woo Jin,Je, Yeon Ho,Choi, Jae Young,Je, Yeon Ho,Li, Shuo. 2017

[9]Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say). Meng, Qing-Wei,Liu, Xin-Ping,Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao. 2015

[10]RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Mu, Li-Li,Chen, Xu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[11]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

[12]Knockdown of an ABC transporter leads to bright red eyes in the brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Delphacidae). Liu, Shu Hua,Wang, Ai Ying,Yang, Bao Jun,Luo, Ju,Tang, Jian. 2017

[13]Efficient RNA interference for three neuronally-expressed genes in Nilaparvata lugens (Stal) (Hemiptera: Delphacidae). Xu, Chen,Guo-Qing, Li,Xu, Chen,Pin-Jun, Wan,Qiang, Fu. 2017

[14]Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens St(a)over-circlel (Hemiptera: Delphacidae). Zhang, Lu,Wang, Huijuan,Chen, Jianyi,Shen, Qida,Wang, Shigui,Tang, Bin,Xu, Hongxing. 2017

[15]Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Ding, Zhiping,Liu, Zewen,Liu, Shuhua,Yang, Baojun,Zhang, Chengwei.

[16]Recent advances in the development of new transgenic animal technology. Miao, Xiangyang,Miao, Xiangyang.

[17]Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference. Cheng, GF,Lin, JJ,Shi, Y,Jin, YX,Fu, ZQ,Jin, YM,Zhou, YC,Cai, YM.

[18]Suppression and restoration of primordial germ cell marker gene expression in channel catfish, Ictalurus punctatus, using knockdown constructs regulated by copper transport protein gene promoters: Potential for reversible transgenic sterilization. Su, Baofeng,Shang, Mei,Peatman, Eric,Perera, Dayan A.,Cheng, Qi,Li, Chao,Weng, Chia-Chen,Li, Ping,Liu, Zhanjiang,Dunham, Rex A.,Su, Baofeng,Shang, Mei,Grewe, Peter M.,Patil, Jawahar G.,Patil, Jawahar G.,Perera, Dayan A.,Li, Chao,Li, Ping.

[19]Two chitinase 5 genes from Locusta migratoria: Molecular characteristics and functional differentiation. Li, Daqi,Zhang, Jianqin,Wang, Yan,Liu, Xiaojian,Ma, Enbo,Sun, Yi,Zhang, Jianzhen,Sun, Yi,Li, Sheng,Zhu, Kun Yan.

[20]RNAi-mediated plant protection against aphids. Yu, Xiu-Dao,Sun, Yong-Wei,Ma, You-Zhi,Xia, Lan-Qin,Yu, Xiu-Dao,Liu, Zong-Cai,Huang, Si-Liang,Chen, Zhi-Qin,Duan, Peng-Fei.

作者其他论文 更多>>

微信小程序