cDNA cloning of a Rab5 homologue in Babesia gibsoni

文献类型: 外文期刊

第一作者: Zhou, JL

作者: Zhou, JL;Ohashi, K;Yamasaki, M;Onuma, M

作者机构:

关键词: Babesia gibsoni;cDNA cloning;Rab5

期刊名称:JOURNAL OF VETERINARY MEDICAL SCIENCE ( 影响因子:1.267; 五年影响因子:1.348 )

ISSN: 0916-7250

年卷期: 2003 年 65 卷 4 期

页码:

收录情况: SCI

摘要: For studying protein trafficking in Babesia-infected erythrocyte, we describe the cloning of a Rab5, one of molecular marker for vesicle trafficking in eukaryotic cells, gene homologue in Babesia gibsoni (BgRab5). The full-length cDNA of BgRab5 is 1,020 bp long with an open reading frame encoding a protein of 220 amino acids. The deduced amino acid sequence of BgRab5 contained the highly conserved GTP-binding consensus sequence and shares about 40% homology with that of Rab5 from Plasmodium falciparum, Toxoplasma gondii, Dog, Lotus japonicusor, Oryza sativa. Northern blot analysis showed that the BgRab5 probe hybridized with a 1 kb band in total RNA from parasitized erythrocytes, that was consistent with the size of the BgRab5 full-length cDNA.

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