Cloning, Characterization, and Functional Expression of Phospholipase D alpha cDNA from Banana (Musa acuminate L.)

文献类型： 外文期刊

第一作者： He, Xuemei

作者： He, Xuemei;Sun, Jian;Li, Changbao;Ling, Dongning;Sheng, Jinfeng;You, Xiangrong;Li, Jiemin;Liu, Guoming;Zheng, Fengjin;Tang, Yayuan;Li, Li;He, Xuemei;Sun, Jian;Li, Changbao

作者机构：

期刊名称：JOURNAL OF FOOD QUALITY （ 影响因子：2.45； 五年影响因子：2.679 ）

ISSN： 0146-9428

年卷期： 2017 年

页码：

收录情况： SCI

摘要： Phospholipase D (PLD) plays a key role in adaptive responses of postharvest fruits. A cDNA clone of banana (Musa acuminate L.) PLD alpha (MaPLD alpha) was obtained by RT-PCR in this study. The MaPLD alpha gene contains a complete open reading frame (ORF) encoding a 92-kDa protein composed of 832 amino acid residues and possesses a characteristic C2 domain and two catalyticHxKxx xD (abbr. HKD) motifs. The two HKD motifs are separated by 341 amino acid residues in the primary structure. Relatively higher PLD activity and expression of MaPLD alpha mRNA were detected in developing tissues compared to senescent or mature tissues in individual leaves, flower, stem, and fruit organs, respectively. The expression profile of PLD alpha mRNA in postharvest banana fruits at different temperatures was determined, and the MaPLD alpha mRNA reached the highest expression peak on day 5 at 25 degrees C and on day 7 at 12 degrees C. The results provide useful information for maintaining postharvest quality and extending the storage life of banana fruit.

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