Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana

文献类型： 外文期刊

第一作者： Liang, Zhaoxu

作者： Liang, Zhaoxu;Wu, Kaichao;He, Shanshan;Jing, Yan;Liang, Jun;Tan, Fang;Li, Song;Li, Ming;Chen, Tuo;Liu, Guangxiu;An, Lizhe;Di, Cuixia;Fang, Weikuan;Zeng, Yuan;Chen, Maoshan;An, Lizhe

作者机构：

期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）

ISSN： 2314-6133

年卷期： 2016 年

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收录情况： SCI

摘要： Background. Water channel proteins, also called a quaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey (C. bungeana) are still unknown. Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana. Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures.

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