Intron targeted amplified polymorphism (ITAP), a new sequence related amplified polymorphism-based technique for generating molecular markers in higher plant species

文献类型： 外文期刊

第一作者： Zhong, Ruichun

作者： Zhong, Ruichun;Jiang, Jing;Han, Zhuqiang;He, Liangqiong;Li, Zhong;Tang, Xiumei;Tang, Ronghua;Xiong, Faqian;Han, Zhuqiang;Tang, Ronghua;Liu, Junxian

作者机构：

关键词： Molecular markers;fingerprinting;genetic diversity;intron targeted amplified polymorphism (ITAP);sequence related amplified polymorphism (SRAP);intron-exon splice junction (ISJ)

期刊名称：PLANT OMICS （ 影响因子：0.777； 五年影响因子：0.802 ）

ISSN： 1836-0661

年卷期： 2013 年 6 卷 2 期

页码：

收录情况： SCI

摘要： Based on the principles of sequence related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP) and conserved region amplification polymorphism (CoRAP), we developed a new molecular marker technique called intron targeted amplified polymorphism (ITAP). For ITAP, primer pairs were exploited to conduct the polymerase chain reaction (PCR). One primer targeting intron was retrieved from the SRAP technique. The other was designed from the 3' widely distributed conserved intron-exon splice junction sequences. Amplification was carried out according to the procedure of the SRAP technique. The PCR products were resolved through standard agarose gel electrophoresis. To test this technique, we have applied it to fingerprint three plant species including banana (Musa spp.), longan (Dimocarpus longan Lour.), and cultivated peanut (Arachis hypogaea L.). Depending upon the different primer pairs, 3-11 bands were detected among these three plant species. Of these bands, 80%, 79.25%, and 45.30% were polymorphic in banana, longan, and cultivated peanut, respectively. Cluster analysis of ITAP markers was largely consistent with previous studies. Since primers were designed from the 3' widely distributed intron-exon splice junction sequences in higher plant species assuming these primers should be universal across other plant species. This technique provides an alternative way to produce molecular markers for plant genotyping and fingerprinting.

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