Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar
文献类型: 外文期刊
第一作者: Shi, YingWu
作者: Shi, YingWu;Li, Chun;Yang, HongMei;Zhang, Tao;Gao, Yan;Chu, Min;Zeng, Jun;Lin, Qing;OuTiKuEr;Li, YuGuo;Huo, Xiangdong;Lou, Kai;Shi, YingWu;Li, Chun;Yang, HongMei;Zhang, Tao;Gao, Yan;Chu, Min;Zeng, Jun;Lin, Qing;OuTiKuEr;Li, YuGuo;Huo, Xiangdong;Lou, Kai
作者机构:
关键词: sugar beet;endophytes;green fluorescent protein;colonization
期刊名称:JOURNAL OF MICROBIOLOGY ( 影响因子:3.422; 五年影响因子:3.28 )
ISSN: 1225-8873
年卷期: 2017 年 55 卷 4 期
页码:
收录情况: SCI
摘要: This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the Achromobacter marplatensis 17:gfp strain in plants 20 days after inoculation, even in the absence of selective pressure, suggests that it is good colonizer. These results also suggest that this strain could be a useful tool for the delivery of enzymes or other proteins into plants. In addition, the study highlights that sugar beet plants can be used effectively for detailed in vitro studies on the interactions between A. marplatensis strain 17 and its host, particularly if a gfp-tagged strain of the pathogen is used.
分类号:
- 相关文献
作者其他论文 更多>>
-
Temperature-driven cotton verticillium wilt: a beta model for risk assessment from laboratory insights to climate scenarios
作者:Zhang, Tianyi;Zhang, Tianyi;Zheng, Bangyou;Xie, Zongming;Xie, Zongming;Zhang, Tao;Feng, Hongjie;Zhou, Jinglong;Ouyang, Fang;Ouyang, Fang
关键词:verticillium wilt; laboratory; Beta; model validation; climate change; China
-
HIGS-mediated crop protection against cotton aphids
作者:Tian, Wen;Zhang, Tao;Zhao, Jian-Hua;Gao, Feng;Wu, Xue-Ming;Zhang, Bo-Sen;Fang, Yuan-Yuan;Guo, Hui-Shan;Tian, Wen;Zhao, Jian-Hua;Guo, Hui-Shan;Dong, Yong-Mei;Li, You-Zhong;Zhao, Zeng-Qiang;Xie, Zong-Ming;Zhao, Jian-Hua
关键词:HIGS; RNAi; cotton aphid; transgenic cotton
-
CircNIPBLL modulates the inflammatory response against Eimeria tenella infection via sponging gga-miR-2954
作者:Yu, Hailiang;Chen, Xingyong;Yu, Hailiang;Chen, Xingyong;Tang, Jianqiang;Dong, Liyue;Tang, Meihui;Arif, Areej;Zhang, Tao;Zhang, Genxi;Xie, Kaizhou;Dai, Guojun;Zhao, Zhenhua
关键词:Chicken; circNIPBLL; Eimeria tenella; Inflammatory response; miRNA
-
Characterization and comparison of metabolites in colostrum from yaks, buffaloes, and cows based on UPLC-QTRAP-MS metabolomics
作者:Zhang, Xueyan;Li, Changhui;Zhang, Hao;Huang, Jiaxiang;Zeng, Qingkun;Li, Ling;Yang, Pan;Wang, Pengjie;Zhang, Hao;Chu, Min;Luo, Jie;Zhang, Hao
关键词:UPLC-MS; Inositol; Carnitine; Primary bile acids; Multivariate statistical analysis; Targeted metabolomics
-
Silencing CaPIP5K4-1 leads to decreased male fertility in Capsicum annuum L.
作者:Kong, Weifu;Wang, Yuhang;Zhang, Tao;Huang, Mianzhu;Kang, Jingtao;Wang, Lina;Wei, Bingqiang;Duan, Panpan;Chang, Yajun
关键词:Male fertility; Pepper; Phosphatidylinositol 4-phosphate 5-kinase; Virus; Induced gene silencing
-
Mapping of resistance genes to powdery mildew based on DNA re-sequencing and bulk segregant analysis in Capsicum
作者:Zhang, Tao;Ma, Yan;Wang, Yuhang;Li, Wei;Kong, Weifu;Zhang, Gaoyuan;Wei, Bingqiang;Bosland, Paul W.;Wei, Min;Duan, Panpan
关键词:BSA-seq; Molecular markers; Pepper; Powdery mildew; Ubiquitin-conjugating enzyme
-
Untargeted Metabolomics Reveals Key Differences Between Yak, Buffalo, and Cow Colostrum Based on UHPLC-ESI-MS/MS
作者:Wang, Yuzhuo;Li, Changhui;Zhang, Hao;Huang, Jiaxiang;Zeng, Qingkun;Li, Ling;Yang, Pan;Wang, Pengjie;Ren, Fazheng;Zhang, Hao;Chu, Min;Luo, Jie;Ren, Fazheng;Zhang, Hao
关键词:untargeted metabolomics; yak colostrum; buffalo colostrum; cow colostrum; specific milk composition; colostrum biomarkers