Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar

文献类型： 外文期刊

第一作者： Shi, YingWu

作者： Shi, YingWu;Li, Chun;Yang, HongMei;Zhang, Tao;Gao, Yan;Chu, Min;Zeng, Jun;Lin, Qing;OuTiKuEr;Li, YuGuo;Huo, Xiangdong;Lou, Kai;Shi, YingWu;Li, Chun;Yang, HongMei;Zhang, Tao;Gao, Yan;Chu, Min;Zeng, Jun;Lin, Qing;OuTiKuEr;Li, YuGuo;Huo, Xiangdong;Lou, Kai

作者机构：

关键词： sugar beet;endophytes;green fluorescent protein;colonization

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN： 1225-8873

年卷期： 2017 年 55 卷 4 期

页码：

收录情况： SCI

摘要： This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the Achromobacter marplatensis 17:gfp strain in plants 20 days after inoculation, even in the absence of selective pressure, suggests that it is good colonizer. These results also suggest that this strain could be a useful tool for the delivery of enzymes or other proteins into plants. In addition, the study highlights that sugar beet plants can be used effectively for detailed in vitro studies on the interactions between A. marplatensis strain 17 and its host, particularly if a gfp-tagged strain of the pathogen is used.

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