Tunable self-assemblies of whey protein isolate fibrils for pickering emulsions structure regulation

文献类型： 外文期刊

第一作者： Jiang, Fangcheng

作者： Jiang, Fangcheng;Huang, Wenjing;Shen, Wangyang;Jin, Weiping;Huang, Qingrong;Jiang, Fangcheng;Pan, Yijun;Huang, Qingrong;Peng, Dengfeng

作者机构： Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China;Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA;Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Oilseeds Proc, Minist Agr, Wuhan, Hubei, Peoples R China

关键词： Self-assembly fibrils; pH-dependent; Small angle X-ray scattering; Pickering emulsions; Confocal-Raman microscopy

期刊名称：FOOD HYDROCOLLOIDS （ 2021影响因子：11.504； 五年影响因子：11.357 ）

ISSN： 0268-005X

年卷期： 2022 年 124 卷

页码：

收录情况： SCI

摘要： Protein fibrils are potential emulsifiers with a highly pH-dependent structure. Therefore, the influence of pH on the self-assemblies of whey protein isolate (WPI) fibrils was investigated through turbidity, transmission electron microscopy, and small-angle X-ray scattering tests. Next, the effects of fibril structure on the interfacial profiles and physical properties of stabilized Pickering emulsions were studied. With an increase in pH from 2.0 to 5.0, the cross-sectional radius of WPI fibrils increased from 1.87 +/- 0.12 nm to 7.75 +/- 0.33 nm, while with a further increase in pH to 7.0, the fibrils decreased to single strand. For all pH conditions, the WPI fibrils effectively absorbed at the oil-water interface. At pH 5.0, the contact angle of fibrils was the maximum (50.6 degrees), and the diffusion rate was the fastest. The d4,3 values of the WPI fibril-stabilized Pickering emulsions were around 70-80 mu m with no significant differences. Moreover, the Pickering emulsions stabilized by fibrils assembled at pH 5.0 showed a predominantly elastic gel-like behavior and high stability. This work clarifies the relationship between the structure of assembled WPI fibrils and their emulsifying properties, and it provides a theoretical basis for developing emulsion-based delivery systems.

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