Immunomodulatory effect of tibetan medicine compound extracts against ORFV in vitro by metabolomics

文献类型： 外文期刊

第一作者： Fan, Yueyuan

作者： Fan, Yueyuan;Wu, Jiao;Li, Saiju;Zeng, Qin;Fu, Guowen;Huang, Wei;Gesang, Zhuoga;Silang, Yuzhen;Zhang, Chong

作者机构：

关键词： ORFV; Tibetan medicine compound; Untargeted metabolomics; Cytokines

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.3； 五年影响因子：2.6 ）

ISSN：

年卷期： 2024 年 20 卷 1 期

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收录情况： SCI

摘要： Ovine contagious pustular dermatitis (ORF) is one of the main diseases of sheep and is a zoonotic disease caused by Ovine contagious pustular dermatitis virus (ORFV) infection, posing a significant constraint on sheep breeding industry and human health. The Tibetan medical formulation composed of Polygonum leucoides, Polygonum xanthoxylum and Acanthophora rotunda significantly regulated lymphocyte immune function following ORFV stimulation, although the mechanism remains unclear. In order to study the immunomodulatory effects and mechanism of three Tibetan medicinal extracts (Polygonum leucoides, Polygonum xanthoxylum, and Acanthophora rotunda) against ORFV in vitro, sheep peripheral blood lymphocytes were isolated in vitro and treated with different concentrations of Tibetan medicine compound extract solution after ORFV infection. The cytokine expression levels in lymphocytes were measured at 4 h, 8 h and 12 h. Additionally endogenous metabolites in lymphocytes at 0 h, 4 h, 8 h and 12 h were quantified by untargeted metabolomics method. The results showed that, the extracts could regulate the lymphocyte immune factors altered by ORFV, and regulate the lymphocyte immune function through cysteine and methionine metabolic pathways as well as the pyrimidine metabolic pathways, potentially alleviating the immune evasion induced by ORFV.

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