Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis

文献类型: 外文期刊

第一作者: Pan, Y

作者: Pan, Y;Cai, H;Li, SX;Tian, X;Li, T;Zhu, YX

作者机构:

关键词: Mycobacterium tuberculosis;secreted proteins;DNA vaccine;protective efficacy

期刊名称:ACTA BIOCHIMICA ET BIOPHYSICA SINICA ( 影响因子:3.848; 五年影响因子:3.67 )

ISSN: 0582-9879

年卷期: 2003 年 35 卷 1 期

页码:

收录情况: SCI

摘要: Three proteins secrected from Mycobacterium tuberculosis, Ag85B, ESAT-6 and MPT63 were seclected as antigens for making combined DNA vaccine by immunizing mice. The immune response induced by the vaccine and its protective efficacy were studied. It was demonstrated that when mice were immunized with the combined DNA vaccine, the titer of antibody for Ag85B in serum increased to more than 10(5), but the titers of ESAT-6 and MPT63 specific antibodies were undetectable. After the final immunization, the level of IFN-gamma specific for Ag85B, ESAT-6 and MPT63 reached (17.0 +/- 7. 0) u/ml, (6.0 +/- 0.8) u/ml and (11.9 +/- 8.0) u/ml, respectively. Mice, that were inoculated with the empty eukaryotic expression vector pJW4303 DNA, produced negligible amounts of antigen-specific INF-gamma. The combined DNA vaccine resulted also in significantly reduced amount of bacteria in the lungs of experimental mice. Microphotographs showed clearly that these lungs were better protected against Mycobacterium tuberculosis challenge than control mice. The combined DNA vaccine reported in this work shed new lights on the prophylactid protection against tuberculosis.

分类号:

  • 相关文献

[1]Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice. Zhao, Yu,Li, Zhong-Yuan,Chen, Jia,Liu, Shan-Shan,Zhu, Xing-Quan,Zhou, Dong-Hui,Zhao, Yu,Sun, Xiao-Lin,Li, Zhong-Yuan,Liu, Shan-Shan,Zhu, Xing-Quan.

[2]Construction and immune effect of Haemophilus parasuis DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mice. Bei, Weicheng,Fu, Shulin,Ou, Jiwen,Tan, Chen,Chen, Huanchun,Bei, Weicheng,Zhang, Minmin,Liu, Jinlin. 2012

[3]Comparative analyses of secreted proteins from the phytopathogenic fungus Verticillium dahliae in response to nitrogen starvation. Jun Chu,Wei-Fang Li,Wang Cheng,Mo Lu,Ke-Hai Zhou,He-Qin Zhu,Fu-Guang Li,Cong-Zhao Zhou.

[4]Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5. Li, Miao,Li, Guoqing,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling.

[5]Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages. Xie, Xiaoli,Han, Meng,Zhang, Liang,Liu, Laixing,Gu, Zuye,Yang, Mei,Yang, Hongjun. 2016

[6]Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Zhang, Yanping,Gao, Yulong,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

[7]Evaluation of two strains of Mareles disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Gao, Yulong,Zhang, Yanping,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

[8]Protective efficacy of a novel recombinant Marek's disease virus vector vaccine against infectious bursal disease in chickens with or without maternal antibodies. Li, Kai,Liu, Yongzhen,Zhang, Yanping,Gao, Li,Liu, Changjun,Cui, Hongyu,Qi, Xiaole,Gao, Yulong,Zhong, Li,Wang, Xiaomei.

[9]Expression of truncated Babesia microti apical membrane protein 1 and rhoptry neck protein 2 and evaluation of their protective efficacy. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.

[10]Protective Efficacy of the Inactivated H5N1 Influenza Vaccine Re-6 Against Different Clades of H5N1 Viruses Isolated in China and the Democratic People's Republic of Korea. Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Liu, Jingli,Guo, Xingfu,Mao, Shenggang,Yang, Fan,Chen, Zhiyu.

[11]Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine. Zheng, Haixue,Lian, Kaiqi,Yang, Fan,Jin, Ye,Zhu, Zixiang,Guo, Jianhong,Cao, Weijun,Liu, Huanan,He, Jijun,Zhang, Keshan,Li, Dan,Liu, Xiangtao.

[12]Prokaryotic expression, identification and bioinformatics analysis of fbpB-esxA fusing gene from Mycobacterium tuberculosis. Wu, Qiang,Zhou, Peng,Wu, Qiang,Fu, Qiongyao,Cai, Qunfang,Fan, Zhigang,Zhan, Zhinong,Niu, Lina,Pei, Hua,Wu, Qiang,Zhou, Peng,Chen, Qian. 2011

[13]Protective Efficacy of an H5N1 Inactivated Vaccine Against Challenge with Lethal H5N1, H5N2, H5N6, and H5N8 Influenza Viruses in Chickens. Tian, Guobin,Chen, Hualan.

[14]Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Yuan, Xianglian,Chen, Liping,Cao, Jun,Yu, Shenye,Liu, Siguo,Yuan, Xianglian,Deng, Xiaoxia,Pang, Hai,Wang Quankai. 2013

[15]Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis. Xu, Lipeng,Liu, Siguo,Xu, Lipeng,Guo, Jiubiao,Zheng, Xiangdong,Pang, Hai,Wen, Tingyi,Sun, Fei. 2010

[16]C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice. Fu, Fang,Tian, Huabin,Li, Xuesong,Lang, Yuekun,Liu, Siguo,Li, Haizhong,Wang, Wei,Li, Xi,Chen, Xin,Li, Xuesong,Tong, Guangzhi. 2013

[17]Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis. Shen, Hongbo,Yang, Yanping,Wang, Feifei,Xu, Shengfeng,Wang, Honghai,Zhang, Ying,Ye, Naihao. 2009

[18]Efficient tuberculosis treatment in mice using chemotherapy and immunotherapy with the combined DNA vaccine encoding Ag85B, MPT-64 and MPT-83. Yu, D-H,Cai, H.,Hu, X-D. 2008

[19]Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. Li, Huixin,Wang, Yulong,Han, Zongxi,Wang, Yu,Liang, Shulin,Jiang, Lu,Kong, Xiangang,Liu, Shengwang,Wang, Yulong,Hu, Yonghao.

[20]Expression, Purification and Characterisation of Secreted Esterase Rv2525c from Mycobacterium tuberculosis. Dang, Guanghui,Chen, Liping,Li, Zhaoli,Cui, Yingying,Cao, Jun,Yu, Shenye,Liu, Siguo,Deng, Xiaoxia,Pang, Hai.

作者其他论文 更多>>