Cloning and sequence analysis of Alcaligenes faecalis nifHDK gene cluster

文献类型： 外文期刊

第一作者： Zhang, HY

作者： Zhang, HY;Lin, M;Xiao, FH;Zhu, HS;Fang, XJ;You, CG;Zhu, YX

作者机构：

关键词： Alcaligenes faecalis nitrogenase;gene cloning;sequence analysis

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Total DNA of Alcaligenes faecalis was probed with both the nifH and nifHD sequences from K. pneumoniae. One positive band of about 4.6 kb was discovered This nifH homologous fragment was cloned into the vector pBluescript SK~+ to construct the recombinant plasmid pBZl. The inserted fragment in pBZl was analyzed by physical mapping and was further subcloned for sequencing. It was found that this A. faecalis nifHDK homology possessed a typical sigma~(54)-dependent promoter region with upstream activator sequence (UAS) and A-T rich region The nifH and nifD ORFs were 888 and 1476 bp long respectively. The GC contents of these two genes were about 61.6% and 60.0%. The intergenic regions of nifH-nifD and nifD-nifK were 101 and 105 bp respectively. There were separate SD sequences upstream of all the three genes. The deduced amino acid sequences of the nifH gene product (the Feprotein) and the nifD gene product (the Mo-Fe-protein) were also highly homologous to other nitrogen-fixing bacteria, especially in those conserved motif. The A faecalis sequence has the highest similarity to that of A vinelandii.

分类号： Q1

  • 相关文献

[1]Molecular Cloning, Sequence Analysis, and Expression of the Polygalacturonase-inhibiting Protein (PGIP) Gene in Mulberry. Hu, Dongqing,Dai, Ruiqiang,Wang, Yuhua,Zhang, Yinghua,Liu, Zhaoyue,Zhao, Weiguo,Hu, Dongqing,Dai, Ruiqiang,Wang, Yuhua,Zhang, Yinghua,Liu, Zhaoyue,Fang, Rongjun,Zhao, Weiguo,Li, Long,Li, Liu,Fang, Rongjun,Lin, Qiang.

[2]Molecular cloning, sequence identification, and gene expression analysis of bovine ADCY2 gene. Li, Y. X.,Seo, K. S.,Jin, H. G.,Yan, C. G.,Jin, X.,Ren, C. Y.,Jiang, C. J.,Jin, C. D..

[3]Cloning and sequence analysis of putative type II fatty acid synthase genes from Arachis hypogaea L.. Li, Meng-Jun,Li, Ai-Qin,Xia, Han,Zhao, Chuan-Zhi,Li, Chang-Sheng,Wan, Shu-Bo,Bi, Yu-Ping,Wang, Xing-Jun. 2009

[4]Identification, cloning and characterization of a novel Gossypium hirsutum L. GhMYBantiV transcription factor in response to biotic and abiotic stresses. Li Fei,Chen Xiaoyan,He Xiaohong,Tang Ming,Hong Kun,Yang Zhengting,Yi Yin,Liu Zhanji,Fu Mingchuan. 2017

[5]Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor. Yang, Qingchuan,Kang, Junmei,Zhang, Tiejun,Sun, Yan,Fang, Feng.

[6]Cloning, Characterization, and Expression Analysis of a Gene Encoding a Putative Lysophosphatidic Acid Acyltransferase from Seeds of Paeonia rockii. Zhang, Qing-Yu,Niu, Li-Xin,Yu, Rui,Zhang, Xiao-Xiao,Bai, Zhang-Zhen,Zhang, Yan-Long,Duan, Ke,Gao, Qing-Hua.

[7]Cloning and differential expression analysis of defensin gene Cldef2.2 from watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Zhang, M.,Yang, X. P.,Xu, J. H.,Liu, G.,Yao, X. F.,Li, P. F.,Zhu, L. L..

[8]Cloning and expression analysis of four DELLA genes in peanut. An, J.,Zhao, Y. X.,Wang, X. J.,An, J.,Hou, L.,Li, C.,Wang, C. X.,Xia, H.,Zhao, C. Z.,Li, C. S.,Wang, X. J.,Li, C.,Zheng, Y. X..

[9]High-level expression and biochemical characterization of a novel cold-active lipase from Rhizomucor endophyticus. Duan, Xiaojie,Liu, Yu,Jiang, Zhengqiang,Yang, Shaoqing,Zheng, Mingming.

[10]Characterization, expression and silencing by RNAi of p53 from Penaeus monodon. Dai, Wenting,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Dai, Wenting,Yang, Qibin.

[11]Expression profiles of selenium dependent glutathione peroxidase and glutathione S-transferase from Exopalaemon carinicauda in response to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei.

[12]cDNA cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Gao, Baoquan,Chen, Ping,Duan, Yafei.

[13]Identification, expression profiling of a grass carp TLR8 and its inhibition leading to the resistance to reovirus in CIK cells. Chen, Xiaohui,Yang, Chunrong,Rao, Youliang,Li, Qingmei,Wan, Quanyuan,Peng, Limin,Su, Jianguo,Wang, Qing,Wu, Shuqin.

[14]A farnesoic acid O-methyltransferase (FAMeT) from Exopalaemon carinicauda is responsive to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun.

[15]Identification and characterization of a gene encoding a putative lysophosphatidyl acyltransferase from Arachis hypogaea. Chen, Si-Long,Huang, Jia-Quan,Lei, Yong,Zhang, Yue-Ting,Ren, Xiao-Ping,Chen, Yu-Ning,Jiang, Hui-Fang,Yan, Li-Ying,Liao, Bo-Shou,Chen, Si-Long,Li, Yu-Rong,Chen, Si-Long.

[16]Mulberry (Morus L.) methionine sulfoxide reductase gene cloning, sequence analysis, and expression in plant development and stress response. Tong, Wei,Zhang, Yinghua,Wang, Heng,Li, Feng,Liu, Zhaoyue,Wang, Yuhua,Fang, Rongjun,Zhao, Weiguo,Li, Long,Wang, Yuhua,Zhao, Weiguo,Li, Long,Fang, Rongjun.

[17]CLONING AND EXPRESSION ANALYSIS OF G-PROTEIN G alpha q SUBUNIT AND G beta 1 SUBUNIT FROM Bemisia tabaci GENNADIUS (HOMOPTERA: ALEYRODIDAE). Tu, Hongtao,Qin, Yuchuan,Tu, Hongtao.

[18]Isolation and Expression Analysis of Fructokinase Genes from Citrus. Qin, QP,Zhang, SL,Chen, JW,Xie, M,Jin, YF,Chen, KS,Asghar, S.

[19]Isolation of laccase gene from Bacillus subtilis and analysis of its physicochemical properties. Yang, Sui-Shan,Liu, Zhen-Wang,Yi, Xiao-Ping,Zhang, Ai-Lian,Zhang, Ze-Hua,Shen, Jin-Cheng,Yin, Hui-Xiang,Chen, Li-Ping,Zhang, Tian-Yuan,Luo, Jin-Xian,Zhang, Tian-Yuan,Luo, Jin-Xian.

[20]Discovery and functional characterization of leptin and its receptors in Japanese quail (Coturnix japonica). Wang, Dandan,Xu, Chunlin,Wang, Taian,Li, Hong,Li, Yanmin,Ren, Junxiao,Tian, Yadong,Li, Zhuanjian,Jiao, Yuping,Kang, Xiangtao,Liu, Xiaojun,Tian, Yadong,Li, Zhuanjian,Kang, Xiangtao,Liu, Xiaojun,Tian, Yadong,Li, Zhuanjian,Kang, Xiangtao,Liu, Xiaojun,Jiao, Yuping.

作者其他论文 更多>>

微信小程序