Cloning and sequence analysis of Alcaligenes faecalis nifHDK gene cluster

文献类型： 外文期刊

第一作者： Zhang, HY

作者： Zhang, HY;Lin, M;Xiao, FH;Zhu, HS;Fang, XJ;You, CG;Zhu, YX

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关键词： Alcaligenes faecalis nitrogenase;gene cloning;sequence analysis

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

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收录情况： SCI

摘要： Total DNA of Alcaligenes faecalis was probed with both the nifH and nifHD sequences from K. pneumoniae. One positive band of about 4.6 kb was discovered This nifH homologous fragment was cloned into the vector pBluescript SK~+ to construct the recombinant plasmid pBZl. The inserted fragment in pBZl was analyzed by physical mapping and was further subcloned for sequencing. It was found that this A. faecalis nifHDK homology possessed a typical sigma~(54)-dependent promoter region with upstream activator sequence (UAS) and A-T rich region The nifH and nifD ORFs were 888 and 1476 bp long respectively. The GC contents of these two genes were about 61.6% and 60.0%. The intergenic regions of nifH-nifD and nifD-nifK were 101 and 105 bp respectively. There were separate SD sequences upstream of all the three genes. The deduced amino acid sequences of the nifH gene product (the Feprotein) and the nifD gene product (the Mo-Fe-protein) were also highly homologous to other nitrogen-fixing bacteria, especially in those conserved motif. The A faecalis sequence has the highest similarity to that of A vinelandii.

分类号： Q1

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