Paper-based immune-affinity arrays for detection of multiple mycotoxins in cereals

文献类型： 外文期刊

第一作者： Li, Li

作者： Li, Li;Chen, Hongpu;Lv, Xiaolan;Wang, Min;Jiang, Xizhi;Jiang, Yifei;Wang, Heye;Zhao, Yongfu;Xia, Liru;Li, Li;Lv, Xiaolan;Chen, Hongpu;Jiang, Yifei

作者机构：

关键词： Paper-based array;Immune-affinity array;Mycotoxins;UV-initiated copolymerization;Chemiluminescent detection

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN： 1618-2642

年卷期： 2018 年 410 卷 8 期

页码：

收录情况： SCI

摘要： Mycotoxins produced by different species of fungi may coexist in cereals and feedstuffs, and could be highly toxic for humans and animals. For quantification of multiple mycotoxins in cereals, we developed a paper-based mycotoxin immune-affinity array. First, paper-based microzone arrays were fabricated by photolithography. Then, monoclonal mycotoxin antibodies were added in a copolymerization reaction with a cross-linker to form an immune-affinity monolith on the paper-based microzone array. With use of a competitive immune-response format, paper-based mycotoxin immune-affinity arrays were successfully applied to detect mycotoxins in samples. The detection limits for deoxynivalenol, zearalenone, T-2 toxin, and HT-2 toxin were 62.7, 10.8, 0.36, and 0.23 mu g center dot kg(-1), respectively, which meet relevant requirements for these compounds in food. The recovery rates were 81-86% for deoxynivalenol, 89-117% for zearalenone, 79-86% for T-2 toxin, and 78-83% for HT-2 toxin, and showed the paper-based immune-affinity arrays had good reproducibility. In summary, the paper-based mycotoxin immune-affinity array provides a sensitive, rapid, accurate, stable, and convenient platform for detection of multiple mycotoxins in agro-foods.

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