Single amino acid in V2 encoded by TYLCV is responsible for its self-interaction, aggregates and pathogenicity

文献类型： 外文期刊

第一作者： Zhao, Wenhao

作者： Zhao, Wenhao;Fan, Yongjian;Zhao, Wenhao;Ji, Yinghua;Wu, Shuhua;Ma, Xiaofang;Li, Shuo;Sun, Feng;Cheng, Zhaobang;Zhou, Yijun;Fan, Yongjian

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： The V2 protein encoded by Begomovirus is essential for virus infection and is involved in multiple functions, such as virus movement and suppression of the host defence response. In this study, we reported that V2 encoded by the Tomato yellow leaf curl virus (TYLCV), which is one of the most devastating tomato-infecting begomoviruses, could interact with itself and a S71A mutation of V2 (V2(S71A)) abolished its self-interaction. Fluorescence results showed that V2 localized primarily in the cytoplasm and around the nucleus. Site-directed mutagenesis V2(S71A) had the similar subcellular localization, but V2(S71A) formed fewer large aggregates in the cytoplasm compared to wild-type V2, whereas the level of aggregates came to a similar after treatment with MG132, which indicates that the S71A mutation might affect 26S proteasome-mediated degradation of V2 aggregates. Meanwhile, heterologous expression of V2(S71A) from a Potato virus X vector induced mild symptoms compared to wild-type V2, delay of virus infection associated with mild symptoms was observed in plants inoculated with TYLCV-S71A, which indicates that the amino acid on position 71 is also involved in the pathogenicity of V2. To the best of our knowledge, this report is the first to state that the S71A mutation of V2 encoded by TYLCV affects the self-interaction, aggregate formation and pathogenicity of V2.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序