Targeted Delivery of GP5 Antigen of PRRSV to M Cells Enhances the Antigen-Specific Systemic and Mucosal Immune Responses
文献类型: 外文期刊
第一作者: Du, Luping
作者: Du, Luping;Yu, Zhengyu;Pang, Fengjiao;Xu, Xiangwei;Mao, Aihua;He, Kongwang;Li, Bin;Du, Luping;Yu, Zhengyu;Pang, Fengjiao;Xu, Xiangwei;Mao, Aihua;He, Kongwang;Li, Bin;Du, Luping;Yuan, Wanzhe
作者机构:
关键词: PRRSV;PLGA;M cells;DNA vaccine;immune response;delivery system
期刊名称:FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY ( 影响因子:5.293; 五年影响因子:5.882 )
ISSN: 2235-2988
年卷期: 2018 年 8 卷
页码:
收录情况: SCI
摘要: Efficient delivery of antigens through oral immunization is a first and critical step for successful induction of mucosal immunity, which can provide protection against pathogens invading the mucosa. Membranous/microfold cells (M cells) within the mucosa can transcytose internalized antigen without degradation and thus play an important role in initiating antigen-specific mucosal immune responses through inducing secretory IgA production. In this research, we modified poly (D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) with Ulex europaeus agglutinin 1 (UEA-1) and successfully prepared an oral vaccine delivery system, UEA-1/PLGA NPs. PLGA NPs were prepared using a standard double emulsion solvent evaporation technique, which can protect the entrapped PRRSV DNA vaccine [pcDNA3.1-SynORF5 (synthetic ORF5)] or subunit vaccine ORF5-encoded glycoprotein (GP5) from exposure to the gastrointestinal (GI) tract and release the plasmids in a controlled manner. With UEA-1 modification, the UEA-1/PLGA NPs can be effectively transported by M-cells. We investigated immune response induced by UEA-1/PLGA-SynORF5 or UEA-1/PLGA-GP5 following inoculation in mice and piglets. Compared with PLGA-SynORF5 or PLGA-GP5 NPs, UEA-1/PLGA-SynORF5, or UEA-1/PLGA-GP5 NPs stimulated significantly increased serum IgG levels and augmented intestinal IgA levels in mice and piglets (P < 0.05). Our findings indicate UEA-1/PLGA NPs can be applied as a promising and universally robust oral vaccine delivery system.
分类号:
- 相关文献
作者其他论文 更多>>
-
The glycosylation sites in RBD of spike protein attenuate the immunogenicity of PEDV AH2012/12
作者:Zhang, Gege;Wang, Jiaxiang;Zhang, Gege;Peng, Qi;Liu, Shiyu;Fan, Baochao;Wang, Chuanhong;Song, Xu;Cao, Qiuxia;Li, Chengcheng;Xu, Hong;Lu, Hongting;Bao, Meiying;Yang, Shanshan;Li, Yunchuan;Li, Bin;Liu, Shiyu;Li, Bin;Liu, Shiyu;Fan, Baochao;Yang, Shanshan;Li, Yunchuan;Li, Bin;Fan, Baochao;Yang, Shanshan;Li, Yunchuan;Li, Bin
关键词:PEDV; RBD; Glycosylation; Reverse genetics; Pathogenicity; Immunogenicity
-
Natural variation of domestication-related genes contributed to latitudinal expansion and adaptation in soybean
作者:Li, Jing;Li, Yecheng;Liu, Yitian;Feng, Yue;Qi, Jie;Li, Bin;Zhang, Shengrui;Sun, Junming;Agyenim-Boateng, Kwadwo Gyapong;Shaibu, Abdulwahab Saliu
关键词:Soybean; Latitudinal expansion; Domestication; Adaptation; Flowering time
-
To improve grape photosynthesis, yield and fruit quality by covering reflective film on the ground of a protected facility
作者:Yuan, Yu;Xie, Yuming;Li, Bin;Wei, Xinyu;Huang, Rutao;Liu, Shuqing;Yuan, Yu;Yuan, Yu;Ma, Lingling
关键词:Reflective film; 'Shine muscat'; Photosynthesis; Grape yield; Fruit quality
-
Analysis of CVC1302-Mediated Enhancement of Monocyte Recruitment in Inducing Immune Responses
作者:Lu, Haiyan;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Li, Lan;Qiao, Xuwen;Cheng, Haiwei;Du, Luping;Chen, Jin;Zheng, Qisheng;Hou, Jibo;Lu, Haiyan;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Li, Lan;Qiao, Xuwen;Cheng, Haiwei;Du, Luping;Chen, Jin;Zheng, Qisheng;Hou, Jibo;Lu, Haiyan;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Li, Lan;Qiao, Xuwen;Cheng, Haiwei;Du, Luping;Chen, Jin;Zheng, Qisheng;Hou, Jibo;Lu, Haiyan;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Li, Lan;Qiao, Xuwen;Cheng, Haiwei;Du, Luping;Chen, Jin;Zheng, Qisheng;Hou, Jibo;Lu, Haiyan
关键词:CVC1302; monocyte; CXCL9; CXCL10; antigen
-
Inhibition of Hyperglycemia and Hyperlipidemia by Blocking Toll-like Receptor 4: Comparison of Wild-Type and Toll-like Receptor 4 Gene Knockout Mice on Obesity and Diabetes Modeling
作者:Zhao, Xingyu;Huang, Wuyang;Zhao, Xingyu;Zheng, Jiawei;Huang, Wuyang;Wang, Jing;Huang, Wuyang;Li, Bin
关键词:TLR4; glycolipid metabolism; obesity and diabetes modeling; knockout
-
Identification of the Effects of 5-Azacytidine on Porcine Circovirus Type 2 Replication in Porcine Kidney Cells
作者:Shan, Yiyi;Wu, Shenglong;Bao, Wenbin;Wu, Zhengchang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Wu, Zhengchang
关键词:5-Azacytidine; porcine circovirus type 2; porcine kidney cells
-
Design and Experiment of an Autonomous Navigation System for a Cattle Barn Feed-Pushing Robot Based on UWB Positioning
作者:Chen, Zejin;Li, Bin;Chen, Zejin;Wang, Haifeng;Zhou, Mengchuang;Zhu, Jun;Chen, Jiahui;Li, Bin
关键词:feed-pushing robot; autonomous navigation system; ultra-wideband; dynamic forward-looking distance; pure pursuit controller