Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus

文献类型： 外文期刊

第一作者： Liu, Zhiyang

作者： Liu, Zhiyang;Li, Xuejuan;Sun, Feng;Zhou, Tong;Zhou, Yijun;Liu, Zhiyang;Li, Xuejuan;Sun, Feng;Zhou, Tong;Zhou, Yijun

作者机构：

关键词： Rice stripe virus;NS3;interaction;OsCIPK30;pathogenesis-related genes

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Rice stripe virus (RSV) causes a severe disease in Oryza sativa (rice) in many Eastern Asian countries. The NS3 protein of RSV is a viral suppressor of RNA silencing, but plant host factors interacting with NS3 have not been reported yet. Here, we present evidence that expression of RSV NS3 in Arabidopsis thaliana causes developmental abnormalities. Through yeast two-hybrid screening and a luciferase complementation imaging assay, we demonstrate that RSV NS3 interacted with OsCIPK30, a CBL (calcineurin B-like proteins)-interaction protein kinase protein. Furthermore, OsCIPK30 was overexpressed to investigate the function of OsCIPK30 in rice. Our investigation showed that overexpression of OsCIPK30 in rice could delay the RSV symptoms and show milder RSV symptoms. In addition, the expression of pathogenesis-related genes was increased in OsCIPK30 transgenic rice. These results suggest that overexpression of OsCIPK30 positively regulates pathogenesis-related genes to enhance the tolerance to RSV in rice. Our findings provide new insight into the molecular mechanism underlying resistance to RSV disease.

分类号：

  • 相关文献

[1]Heat shock protein 70 is necessary for Rice stripe virus infection in plants. Jiang, Shanshan,Li, Kunfeng,Lin, Lin,Zheng, Hongying,Chen, Jianping,Jiang, Shanshan,Lu, Yuwen,Li, Kunfeng,Yan, Fei,Chen, Jianping.

[2]Characterization of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) helicase activity and its modulation by CSFV RNA-dependent RNA polymerase. Wen, Guoyuan,Xue, Jinnuo,Shen, Yanping,Zhang, Chuyu,Pan, Zishu,Wen, Guoyuan. 2009

[3]Characterization and Genetic Analysis of a Novel Rice Spotted-leaf Mutant HM47 with Broad-spectrum Resistance to Xanthomonas oryzae pv. oryzae. Feng, Bao-Hua,Yang, Yang,Shi, Yong-Feng,Shen, Hai-Chao,Wang, Hui-Mei,Huang, Qi-Na,Xu, Xia,Lu, Xiang-Guang,Wu, Jian-Li,Yang, Yang. 2013

[4]Differential expression profiling of the early response to Ustilaginoidea virens between false smut resistant and susceptible rice varieties. Han, Yanqing,Zhang, Kang,Yang, Jun,Zhang, Nan,Fang, Anfei,Zhang, Yong,Sun, Wenxian,Han, Yanqing,Zhang, Kang,Yang, Jun,Zhang, Nan,Fang, Anfei,Zhang, Yong,Sun, Wenxian,Liu, Yongfeng,Chen, Zhiyi,Hsiang, Tom. 2015

[5]Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference. Ma, Jin,Song, Yunzhi,Wu, Bin,Li, Kaidong,Zhu, Changxiang,Wen, Fujiang,Jiang, Mingsong. 2011

[6]Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Jiang, Yayuan,Sun, Lin,Li, Kaidong,Song, Yunzhi,Zhu, Changxiang,Jiang, Mingsong.

[7]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

[8]Dimeric artificial microRNAs mediate high resistance to RSV and RBSDV in transgenic rice plants. Sun, Lin,Lin, Chao,Du, Jinwen,Song, Yunzhi,Liu, Hongmei,Zhou, Shumei,Wen, Fujiang,Zhu, Changxiang,Jiang, Mingsong.

[9]Inheritance of resistance to rice stripe virus in rice line 'BL 1'. Ise, K,Ishikawa, K,Li, CY,Ye, CR. 2002

[10]Rice Stripe Virus Interferes with S-acylation of Remorin and Induces Its Autophagic Degradation to Facilitate Virus Infection. Xu, Yi,Li, Chenyang,Wu, Jianxiang,Zhou, Xueping,Li, Chenyang,Zhou, Xueping,Li, Yi,Xu, Yi. 2018

[11]Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. Xiong, Ruyi,Wu, Jianxiang,Zhou, Xueping,Zhou, Yijun. 2009

[12]RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus. Fang, Ying,Lee, Seok Hee,Kim, Jong Hoon,Park, Dong Hwan,Park, Min Gu,Woo, Ra Mi,Lee, Bo Ram,Kim, Woo Jin,Je, Yeon Ho,Choi, Jae Young,Je, Yeon Ho,Li, Shuo. 2017

[13]Over-expression of Oryza sativa Xrn4 confers plant resistance to virus infection. Jiang, Shanshan,Jiang, Liangliang,Jiang, Shanshan,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei. 2018

[14]Arabidopsis is Susceptible to Rice stripe virus Infections. Sun, Feng,Yuan, Xia,Zhou, Tong,Fan, Yongjian,Zhou, Yijun,Yuan, Xia. 2011

[15]RNA-seq-based digital gene expression analysis reveals modification of host defense responses by rice stripe virus during disease symptom development in Arabidopsis. Sun, Feng,Fang, Peng,Li, Juan,Du, Linlin,Lan, Ying,Zhou, Tong,Fan, Yongjian,Zhou, Yijun,Fang, Peng,Shen, Wenbiao. 2016

[16]Altered accumulation of osa-miR171b contributes to rice stripe virus infection by regulating disease symptoms. Tong, Aizi,Yuan, Quan,Wang, Shu,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei,Tong, Aizi,Chen, Hairu,Yuan, Quan,Gong, Yifu,Wang, Shu. 2017

[17]Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy. Deng, Jinhua,Li, Shuo,Ji, Yinghua,Zhou, Yijun,Deng, Jinhua,Hong, Jian. 2013

[18]Transcription of ORFs on RNA2 and RNA4 of Rice stripe virus terminate at an AUCCGGAU sequence that is conserved in the genus Tenuivirus. Wu, Gentu,Wu, Gentu,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Yan, Fei,Chen, Jianping.

[19]Expression of defense genes and activities of antioxidant enzymes in rice resistance to rice stripe virus and small brown planthopper. Hao, Zhongna,Wang, Lianping,He, Yueping,Liang, Jiangen,Tao, Rongxiang.

[20]The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses. Lin, Wenzhong,Qiu, Ping,Jin, Jing,Liu, Shunmin,Ul Islam, Saif,Zhang, Jie,Du, Zhenguo,Wu, Zujian,Yang, Jinguang,Kormelink, Richard,Du, Zhenguo,Wu, Zujian. 2017

作者其他论文 更多>>

微信小程序