Simultaneous production of monoclonal antibodies against Bacillus thuringiensis (Bt) Cry1 toxins using a mixture immunization

文献类型： 外文期刊

第一作者： Dong, Sa

作者： Dong, Sa;Zhang, Cunzheng;Liu, Yuan;Zhang, Xiao;Xie, Yajing;Zhong, Jianfeng;Xu, Chongxin;Liu, Xianjin;Dong, Sa;Liu, Xianjin

作者机构：

关键词： Mixture immunization;Cry1 family;Diverse hybridomas;Monoclonal antibody;DAS-ELISA

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN： 0003-2697

年卷期： 2017 年 531 卷

页码：

收录情况： SCI

摘要： The detections of Cry1 toxins are mainly dependent on immunoassays based on specific monoclonal antibodies (mAb). In the present study, a mixture immunization with seven Cry1 toxins was administered. The results showed that five mAbs with different characteristics, especially one mAb named 5-E8 which could recognize all the seven Cry1 toxins were obtained. Based on the 5-E8 mAb, a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) which can specifically detect the seven Cry1 toxins without cross-reactivity to Cry2A and vip3 was developed with the limit of detection (LOD) and limit of quantification (LOQ) of 6.37-11.35 ng mL(-1) and 17.36-24.48 ng mL(-1), respectively. The recovery tests showed that the recoveries ranged from 78% to 110% within the quantitation range (LOQ-100 ng mL(-1)). The established DAS-ELISA can be a useful tool for monitoring the Cry1 toxins in agricultural products. Mixture immunization opens a new path for producing diverse mAbs simultaneously in a single immunization circle. (C) 2017 Elsevier Inc. All rights reserved.

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