Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

文献类型： 外文期刊

第一作者： Sun, Xiaochuan

作者： Sun, Xiaochuan;Wang, Yan;Xu, Liang;Li, Chao;Zhang, Wei;Luo, Xiaobo;Jiang, Haiyan;Liu, Liwang;Sun, Xiaochuan;Sun, Xiaochuan;Wang, Yan;Xu, Liang;Luo, Xiaobo;Liu, Liwang

作者机构：

关键词： radish;salt stress;iTRAQ;proteomics;association analysis

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.

分类号：

  • 相关文献

[1]Identification of early salt stress responsive proteins in seedling roots of upland cotton (Gossypium hirsutum L.) employing iTRAQ-based proteomic technique. Li, Wu,Li, Wu,Zhao, Fu'an,Fang, Weiping,Xie, Deyi,Hou, Jianan,Yang, Xiaojie,Zhao, Yuanming,Tang, Zhongjie,Nie, Lihong,Lv, Shuping. 2015

[2]iTRAQ-proteomics and bioinformatics analyses of mammary tissue from cows with clinical mastitis due to natural infection with Staphylococci aureus. Huang, Jinming,Luo, Guojing,Zhang, Zijing,Wang, Xiuge,Ju, Zhihua,Qi, Chao,Zhang, Yan,Wang, Changfa,Li, Rongling,Li, Jianbin,Yin, Weijun,Zhong, Jifeng,Luo, Guojing,Zhang, Zijing,Xu, Yinxue,Moisa, Sonia J.,Loor, Juan J.,Loor, Juan J.,Moisa, Sonia J.,Loor, Juan J.. 2014

[3]iTRAQ Protein Profile Differential Analysis between Somatic Globular and Cotyledonary Embryos Reveals Stress, Hormone, and Respiration Involved in Increasing Plant let Regeneration of Gossypium hirsutum L.. Xiaoyang Ge,Chaojun Zhang,Qianhua Wang,Zuoren Yang,Ye Wang,Xueyan Zhang,Zhixia Wu,Yuxia Hou,Jiahe Wu,Fuguang Li.

[4]Identification of cold-stress responsive proteins in Anabasis aphylla seedlings via the iTRAQ proteomics technique. Wang, Tingting,Wang, Mei,Chu, Guangming,Ye, Chunxiu,Ye, Chunxiu. 2017

[5]iTRAQ-based proteomic profiling of granulosa cells from lamb and ewe after superstimulation. Lin, Jiapeng,Lin, Jiapeng,Wu, Yangsheng,Han, Bing,Chen, Ying,Wang, Liqin,Li, Xiaolin,Liu, Mingjun,Huang, Juncheng. 2017

[6]iTRAQ-based quantitative proteomic analysis of wheat roots in response to salt stress. Jiang, Qiyan,Niu, Fengjuan,Sun, Xianjun,Hu, Zheng,Zhang, Hui,Li, Xiaojuan.

[7]iTRAQ Protein Profiling of Adventitious Root Formation in Mulberry Hardwood Cuttings. Tang, Zhuang,Du, Wei,Du, XiaoLong,Ban, YueYuan,Cheng, JiaLing,Du, Wei,Cheng, JiaLing.

[8]Proteomic Analysis Reveals Resistance Mechanism Against Chlorpyrifos in Frankliniella occidentalis (Thysanoptera: Thripidae). Yan, Dan-Kan,Hu, Min,Tang, Yun-Xia,Fan, Jia-Qin,Yan, Dan-Kan,Hu, Min,Tang, Yun-Xia,Fan, Jia-Qin,Yan, Dan-Kan.

[9]Proteomic analysis of differentially expressed proteins in the three developmental stages of Trichinella spiralis. Liu, J. Y.,Zhang, N. Z.,Li, W. H.,Li, L.,Yan, H. B.,Qu, Z. G.,Li, T. T.,Cui, J. M.,Yang, Y.,Jia, W. Z.,Fu, B. Q.,Jia, W. Z.,Fu, B. Q..

[10]iTRAQ-based proteomic study of the effects of Spiroplasma eriocheiris on Chinese mitten crab Eriocheir sinensis hemocytes. Meng, Qingguo,Hou, Libo,Zhao, Yang,Huang, Xin,Gu, Wei,Wang, Wen,Meng, Qingguo,Hou, Libo,Zhao, Yang,Huang, Xin,Gu, Wei,Wang, Wen,Huang, Yanqing,Xia, Siyao.

[11]Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation. Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Zhu, Xing-Quan,He, Shuai,Zhou, Dong-Hui,Elsheikha, Hany M.,He, Shuai,Li, Qian.

[12]Analyses of the Molecular Mechanisms Associated with Silk Production in Silkworm by iTRAQ-Based Proteomics and RNA-Sequencing-Based Transcriptomics. Wang, Shaohua,You, Zhengying,Che, Jiaqian,Zhang, Yuyu,Qian, Qiujie,Zhong, Boxiong,Feng, Mao,Komatsu, Setsuko.

[13]Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns. Zhou, Dong-Hui,Wang, Ze-Xiang,Zhou, Chun-Xue,He, Shuai,Zhu, Xing-Quan,Elsheikha, Hany M.,Zhou, Chun-Xue,He, Shuai. 2017

[14]Proteomic Differences between Developmental Stages of Toxoplasma gondii Revealed by iTRAQ-Based Quantitative Proteomics. Wang, Ze-Xiang,Zhou, Chun-Xue,He, Shuai,Zhou, Dong-Hui,Zhu, Xing-Quan,Zhou, Chun-Xue,Zhou, Chun-Xue,Elsheikha, Hany M.,He, Shuai,Zhu, Xing-Quan. 2017

[15]Comparative Proteomic Analysis Provides insight into the Key Proteins as Possible Targets Involved in Aspirin Inhibiting Biofilm Formation of Staphylococcus xylosus. Xu, Chang-Geng,Yang, Yan-Bei,Zhou, Yong-Hui,Hao, Mei-Qi,Ren, Yong-Zhi,Wang, Xiao-Ting,Chen, Jian-Qing,Muhammad, Ishfaq,Wang, Shuai,Li, Yan-Hua,Liu, Di,Li, Xiu-Bo,Li, Yan-Hua. 2017

[16]Comparative proteomic analysis of latex from Hevea brasiliensis treated with Ethrel and methyl jasmonate using iTRAQ-coupled two-dimensional LC-MS/MS. Zeng, Rizhong.

[17]iTRAQ-based quantitative proteomic analysis of Macrobrachium rosenbergii hemocytes during Spiroplasma eriocheiris infection. Hou, Libo,Xiu, Yunji,Wang, Jian,Liu, Yuhan,Gu, Wei,Wang, Wen,Meng, Qingguo,Xiu, Yunji,Liu, Xiaoqian,Hou, Libo,Xiu, Yunji,Wang, Jian,Liu, Yuhan,Gu, Wei,Wang, Wen,Meng, Qingguo.

[18]iTRAQ-based comparative proteomic analysis reveals tissue-specific and novel early-stage molecular mechanisms of salt stress response in Carex rigescens. Li, Mingna,Zhang, Kun,Sun, Yan,Cao, Shihao,Long, Ruicai,Kang, Junmei,Zhang, Tiejun. 2017

[19]Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination. Ma, Qiaoli,Kang, Junmei,Zhang, Kun,Wang, Tenghua,Sun, Yan,Kang, Junmei,Long, Ruicai,Zhang, Tiejun,Yang, Qingchuan,Ma, Qiaoli,Xiong, Junbo.

[20]Proteomics reveals the effects of gibberellic acid (GA(3)) on salt-stressed rice (Oryza sativa L.) shoots. Wen, Fu-ping,Zhang, Zhao-hui,Bai, Ting,Xu, Qin,Pan, Ying-hong. 2010

作者其他论文 更多>>

微信小程序