Biochemical characterization of an enantioselective esterase from Brevundimonas sp LY-2

文献类型： 外文期刊

第一作者： Zhang, Jing

作者： Zhang, Jing;Zhao, Mengjun;Yu, Die;Zhang, Hao;Huang, Xing;Zhang, Jing;Yin, Jingang

作者机构：

关键词： Esterase;Lactofen;Brevundimonas sp.;Enantioselective degradation

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：5.328； 五年影响因子：5.588 ）

ISSN： 1475-2859

年卷期： 2017 年 16 卷

页码：

收录情况： SCI

摘要： Background: Lactofen, a member of the diphenylether herbicides, has high activity and is commonly used to control broadleaf weeds. As a post-emergent herbicide, it is directly released to the environment, and easily caused the pollution. This herbicide is degraded in soil mainly by microbial activity, but the functional enzyme involved in the biodegradation of lactofen is still not clear now. Results: A novel esterase gene lacH, involved in the degradation of lactofen, was cloned from the strain Brevundimonas sp. LY-2. The gene contained an open reading frame of 921 bp, and a putative signal peptide at the N-terminal was identified with the most likely cleavage site between Ala 28 and Ala 29. The encoded protein, LacH, could catalyze the hydrolysis of lactofen to form acifluorfen. Phylogenetic analysis showed that LacH belong to family V of bacterial lipolytic enzymes. Biochemical characterization analysis showed that LacH was a neutral esterase with an optimal pH of 7.0 and an optimal temperature of 40 degrees C toward lactofen. Besides, the activity of LacH was strongly inhibited by Hg2+ and Zn2+. LacH preferred short chain p-nitrophenyl esters (C-2-C-6), exhibited maximum activity toward p-nitrophenyl acetate. Furthermore, the enantioselectivity of LacH during lactofen hydrolysis was also studied, and the results show that R-(-)-lactofen was degraded faster than S-(+)-lactofen, indicating the occurrence of enantioselectivity in the enzymatic reaction. Conclusions: Our studies characterized a novel esterase involved in the biodegradation of diphenylether herbicide lactofen. The esterase showed enantioselectivity during lactofen degradation, which revealed the occurrence of enzyme-mediated enantioselective degradation of chiral herbicides.

分类号：

  • 相关文献

[1]Detoxification of diphenyl ether herbicide lactofen by Bacillus sp Za and enantioselective characteristics of an esterase gene lacE. Zhang, Jing,Lu, Luyao,Chen, Feng,Chen, Lingling,Huang, Xing,Zhang, Jing,Yin, Jingang. 2018

[2]Influence of Soil Properties on the Enantioselective Dissipation of the Herbicide Lactofen in Soils. Diao, Jinling,Lv, Chunguang,Dang, Ziheng,Zhu, Wentao,Zhou, Zhiqiang,Wang, Xinquan.

[3]Interaction of plant epicuticular waxes and extracellular esterases of Curvularia eragrostidis during infection of Digitaria sanguinalis and Festuca arundinacea by the fungus. Wang, Fei,Zhang, Peng,Qiang, Sheng,Xu, Lang-Lai. 2006

[4]Low Temperature Storage of Eggs Improve the Development and Reproduction of Locusta migratoria (Orthoptera: Acrididae). Jia, Miao,Zhao, Xia,Wang, Lei,Tu, Xiongbing,Wang, Guangjun,Nong, Xiangqun,Zhang, Zehua.

[5]Dwarf 88, a novel putative esterase gene affecting architecture of rice plant. Gao, Zhenyu,Liu, Xiaohui,Feng, Qi,Han, Bin,Gao, Zhenyu,Qian, Qian,Yan, Meixian,Dong, Guojun,Liu, Jian.

[6]Molecular cloning and characterization of a novel SGNH arylesterase from the goat rumen contents. Wang, Guozeng,Meng, Kun,Luo, Huiying,Wang, Yaru,Huang, Huoqing,Shi, Pengjun,Pan, Xia,Yang, Peilong,Yao, Bin.

[7]A novel chlorpyrifos hydrolase CPD from Paracoccus sp TRP: Molecular cloning, characterization and catalytic mechanism. Fan, Shuanghu,Yan, Yanchun,Wang, Junhuan,Wang, Jiayi,Qiao, Cheng,Yang, Ting,Jia, Yang,Zhao, Baisuo,Li, Kang. 2018

[8]Mechanisms for multiple resistances in field populations of common cutworm, Spodoptera litura (Fabricius) in China. Huang, Shuijin,Han, Zhaojun. 2007

[9]PlantcellwallpolysaccharidesdeconstructionbyCaldicellulosiruptorbescii. 苏小运. 2015

[10]Enantioselective determination of triazole fungice tetraconazole by chiral high-performance liquid chromatography and its application to pharmacokinetic study in cucumber, muskmelon, and soils. Dong, Fengshou,Xu, Jun,Liu, Xingang,Li, Yuanbo,Zheng, Yongquan,Shan, Weili.

[11]Enantioselective determination of the insecticide indoxacarb in cucumber and tomato by chiral liquid chromatography-tandem mass spectrometry. Dong, Fengshou,Li, Yuanbo,Chen, Xiu,Zheng, Yongquan,Wang, Huanqing,Xiang, Wensheng,Cheng, Youpu.

[12]Enantioselective Degradation and Enantiomerization of Indoxacarb in Soil. Sun, Dali,Liu, Chenglan,Sun, Dali,Pang, Junxiao,Jiao, Bining,Qiu, Jing,Li, Li.

[13]Stereoselective separation and pharmacokinetic dissipation of the chiral neonicotinoid sulfoxaflor in soil by ultraperformance convergence chromatography/tandem mass spectrometry. Dong, Fengshou,Xu, Jun,Liu, Xingang,Cheng, Youpu,Liu, Na,Tao, Yan,Pan, Xinglu,Zheng, Yongquan.

[14]Enantioselective separation and pharmacokinetic dissipation of cyflumetofen in field soil by ultra-performance convergence chromatography with tandem mass spectrometry. Dong, Fengshou,Xu, Jun,Liu, Xingang,Chen, Zenglong,Pan, Xinglu,Chen, Xixi,Zheng, Yongquan,Liu, Na.

[15]Enantioselective Degradation of Chiral Insecticide Dinotefuran in Greenhouse Cucumber and Soil. Dong, Fengshou,Xu, Jun,Liu, Xingang,Wang, Yunhao,Zheng, Yongquan,Chen, Xiu.

[16]Rapid Enantioseparation and Determination of Isocarbophos Enantiomers in Orange Pulp, Peel, and Kumquat by Chiral HPLC-MS/MS. Qi, Peipei,Wang, Xiangyun,Zhang, Hu,Wang, Xinquan,Xu, Hao,Wang, Qiang,Qi, Peipei,Wang, Xiangyun,Zhang, Hu,Wang, Xinquan,Xu, Hao,Wang, Qiang,Qi, Peipei,Wang, Xiangyun,Zhang, Hu,Wang, Xinquan,Xu, Hao,Wang, Qiang,Qi, Peipei,Wang, Xiangyun,Zhang, Hu,Wang, Xinquan,Xu, Hao,Wang, Qiang.

[17]Stereoselective Analysis and Degradation of Pyrisoxazole in Cabbage, Pakchoi, and Pepper by Liquid Chromatography Tandem Mass Spectrometry. Yang, Xuewei,Sun, Yuhan,Wang, Lidong,Zhao, Changshan,Qi, Peipei,Wang, Xiangyun,Wang, Zhiwei,Xu, Xiahong,Xu, Hao,Wang, Qiang,Wang, Xinquan,Qi, Peipei,Wang, Xiangyun,Wang, Zhiwei,Xu, Xiahong,Xu, Hao,Wang, Qiang,Wang, Xinquan,Qi, Peipei,Wang, Xiangyun,Wang, Zhiwei,Xu, Xiahong,Xu, Hao,Wang, Qiang,Wang, Xinquan.

[18]Enantioselective Degradation of (2RS, 3RS)-Paclobutrazol in Rat Liver Microsomes. Wu, Shuchun,Han, Jianzhong,Wu, Shuchun,Yu, Miao,Zhang, Hu.,Qian, Mingrong.

[19]Enantioselective Degradation of (2RS,3RS)-Paclobutrazol in Peach and Mandarin Under Field Conditions. Wang, Xiangyun,Qi, Peipei,Yang, Guiling,Wang, Xinquan,Zhang, Hu,Xu, Hao,Wang, Zhiwei,Wang, Qiang,Wang, Xiangyun,Qi, Peipei,Yang, Guiling,Wang, Xinquan,Zhang, Hu,Xu, Hao,Wang, Zhiwei,Wang, Qiang,Wang, Xiangyun,Qi, Peipei,Yang, Guiling,Wang, Xinquan,Zhang, Hu,Xu, Hao,Wang, Zhiwei,Wang, Qiang.

作者其他论文 更多>>

微信小程序