Signal-Amplified Lateral Flow Test Strip for Visual Detection of Cu2+

文献类型： 外文期刊

第一作者： Wang, Yulong

作者： Wang, Yulong;Liu, Fengquan;Wang, Limin;Xue, Juanjuan;Dong, Jinbo;Cai, Jia;Hua, Xiude;Wang, Minghua;Liu, Fengquan;Zhang, Cunzheng

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 1 期

页码：

收录情况： SCI

摘要： A signal-amplified lateral flow test strip (SA-LFTS) for the detection of Cu2+ in aqueous solution was constructed based on Cutcatalyzed click chemistry and hybridization of single stranded DNA (ssDNA). Alkyne and azide modified ssDNA acted as specific elements for Cu2+ recognition, and a chemical ligation product formed through Cutcatalyzed alkyneazide cycloaddition. Hybridization of ssDNA-labeled gold nanoparticles resulted in high sensitivity, and the output signal could be observed directly by the naked eye. Using the developed SA-LFTS under optimal conditions, Cu2+ could be detected rapidly with limit of detections of 5 nM and 4.2 nM by visual observation and quantitative analysis, respectively. The sensitivity (i.e. the visual limit of detection) of the SA-LFTS was 80-times higher than that of traditional LFTS. The SA-LFTS was applied to the determination of Cu2+ in municipal water and river water samples with the results showing good recovery and accuracy. The developed test strip is promising for point-of-care applications and detection of Cu2+ in the field.

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