Transcriptional analysis of porcine circovirus-like virus P1

文献类型： 外文期刊

第一作者： Wen, Li-bin

作者： Wen, Li-bin;Wang, Feng-zhi;He, Kong-wang;Li, Bin;Wang, Xiao-min;Guo, Rong-li;Xie, Jian-ping

作者机构：

关键词： Porcine circovirus like virus P1;Transcriptional analysis;Northern blotting;RACE

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2014 年 10 卷

页码：

收录情况： SCI

摘要： Background: Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts. Results: The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids). Conclusions: We show a very complex viral transcription pattern in P1-infected cells.

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