Development of Phage Immuno-Loop-Mediated Isothermal Amplification Assays for Organophosphorus Pesticides in Agro-products

文献类型： 外文期刊

第一作者： Hua, Xiude

作者： Hua, Xiude;Yin, Wei;Shi, Haiyan;Li, Ming;Ye, Yonghao;Wang, Minghua;Hua, Xiude;Yin, Wei;Shi, Haiyan;Li, Ming;Ye, Yonghao;Wang, Minghua;Wang, Yanru;Kim, Hee Joo;Gee, Shirley J.;Hammock, Bruce D.;Wang, Yanru;Kim, Hee Joo;Gee, Shirley J.;Hammock, Bruce D.;Wang, Hong;Liu, Fengquan

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

ISSN： 0003-2700

年卷期： 2014 年 86 卷 16 期

页码：

收录情况： SCI

摘要： Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.

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