Effect of FTO Expression and Polymorphism on Fat Deposition in Suzhong Pigs

文献类型: 外文期刊

第一作者: Fu, Yanfeng

作者: Fu, Yanfeng;Ren, Shouwen;Lil, Lan;Lil, Lan

作者机构:

关键词: FTO;Fat Deposition;Expression;Polymorphism;Suzhong Pig

期刊名称:ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES ( 影响因子:2.509; 五年影响因子:2.604 )

ISSN: 1011-2367

年卷期: 2013 年 26 卷 10 期

页码:

收录情况: SCI

摘要: Fat mass and obesity associated gene (FTO) plays an important role in appetite control and energy consumption in human and mice. In order to examine FTO expression influence on fat deposition in Suzhong pigs, FTO mRNA expression was detected in 16 tissues by RT-PCR, FTO protein expression was detected in 5 tissues by western blot, and association of FTO polymorphism with meat quality traits was analyzed in Suzhong populations with 714 records. RT-PCR results revealed that FTO mRNA was expressed in all sixteen tissues with significant differences (p<0.05), expression in backfat was significantly higher than that of any other tissue (p<0.05), and expression in longissimus dorsi muscle had the second highest significance level (p<0.05). Western blot results demonstrated that FTO protein was highly expressed in backfat and longissimus dorsi muscle. Furthermore, FTO mRNA and protein expression in tissues of high-fat pigs was significantly higher than that of low-fat pigs (p<0.05), suggesting FTO expression had advantageous effects on fat deposition. FTO polymorphism results evidenced that at A227G locus, G allele seemed to have advantageous effects on fat deposition, indicating it could be a significant candidate gene for improving pork quality in Suzhong pigs.

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