Improvement of transfection efficiency by galactosylated N-3-guanidinopropyl methacrylamide-co-poly (ethylene glycol) methacrylate copolymers

文献类型： 外文期刊

第一作者： Li, Xinsong

作者： Li, Xinsong;Wu, Yang;Liu, Wei;Li, Ling;Guo, Liang;Li, Yunhui;Yin, Lihong;Pu, Yuepu;Qin, Zhu

作者机构：

关键词： Gene delivery;Guanidinylated;Cell penetration;Hepatocyte targeting;Poly (ethylene glycol) methaceylate

期刊名称：EUROPEAN POLYMER JOURNAL （ 影响因子：4.598； 五年影响因子：4.674 ）

ISSN： 0014-3057

年卷期： 2013 年 49 卷 10 期

页码：

收录情况： SCI

摘要： GalactosylatedN-3-guanidinopropylmethacrylamide-co-poly (ethylene glycol) methacrylate copolymers (galactosylated GPMA-co-PEGMA, GGP) were developed in order to promote transfection efficiency in the presence of serum in this report. First of all, the galactosylated PEGMA-co-GPMA copolymers were prepared via aqueous reversible addition fragmentation chain transfer polymerization (RAFT) of poly (ethylene glycol) methacrylate (PEGMA) with long circulating chain segment and N-3-aminopropyl methacrylamide (APMA) followed by galactosylation and guanidinylation. After that, GGP/plasmid DNA complexes were examined by a dynamic light scattering and gel electrophoresis. It is showed that GGP copolymers have effective condensing ability. The cytotoxicity of GGP was measured by MTT assay. It was found that all the GGP/plasmid DNA complexes had less cytotoxic effects on HepG2 cells than HeLa cells, and the galactose groups reduced the cytotoxicity of complexes with high charge ratios to HepG2 cells. Finally, the transfection efficiency of the galactosylated PEGMA-co-GPMA copolymers was investigated by luciferase expression assay. The results revealed that the copolymers with galactose groups more than 5.83% could induce the asialoglycoprotein (ASGP) receptor mediated transfection, which improved the transfection efficiency in target cells. The GPMA-co-PEGMA copolymers with 54.57% hydrophilic chain segment PEG should prevent the aggregation of protein on the GGP/pDNA complexes, and GGP with 7.94% galactose graft exhibited the highest transfection in the presence of serum. (C) 2013 Elsevier Ltd. All rights reserved.

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