Six Alkaloids Inhibit Secretion of IL-1 alpha, TXB2, ET-1 and E-selectin in LPS-induced Endothelial Cells

文献类型: 外文期刊

第一作者: Hu, Yi-Yi

作者: Hu, Yi-Yi;He, Kong-Wang;Guo, Rong-li

作者机构:

关键词: IL-1 alpha;TXB2;ET-1;E-selectin;LPS;Alkaloid

期刊名称:IMMUNOLOGICAL INVESTIGATIONS ( 影响因子:3.657; 五年影响因子:3.279 )

ISSN: 0882-0139

年卷期: 2012 年 41 卷 3 期

页码:

收录情况: SCI

摘要: The aim of the research was to investigate the antiendotoxin effects of Sinomenine, Fangchinoline, Stachydrine, Chuanxionggzine, Oxymartrine and Evodiamine. Endothelial cells were challenged with 1 mu g/mL LPS for 3 h then treated respectively with six alkaloids at three concentrations ( 1, 5 and 10 mu g/mL). The cells were incubated at 37 degrees C in a cell incubator for 21 h. The supernatants were collected and analyzed the levels of interleukin-1 alpha (IL-1 alpha), thromboxane B-2 (TXB2), endothelin-1 (ET-1) and E-selectin by ELISA kits. The results revealed that Sinomenine, Oxymartrine and Evodiamine inhibited the production of IL-1 alpha; Stachydrine, Chuanxionggzine and Evodiamine inhibited the secretion of TXB2; Sinomenine and Oxymartrine down-regulated ET-1 expression; Fangchinoline and Evodiamine decreased the level of E-selectin. All these changes were significant. Taken together, the data suggested that six alkaloids may effectively reduce inflammatory response via these cytokines.

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