Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses

文献类型： 外文期刊

第一作者： Xu, Dao-kun

作者： Xu, Dao-kun;Jin, Qi-jiang;Xie, Yan-jie;Liu, Ya-hui;Lin, Yu-ting;Shen, Wen-biao;Zhou, Yi-jun

作者机构：

关键词： abiotic stress responses;carbon monoxide;heme oxygenase-1;prokaryotic expression in E. coli;wheat

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2011 年 12 卷 11 期

页码：

收录情况： SCI

摘要： In animals and recently in plants, heme oxygenase-1 (HO1) has been found to confer protection against a variety of oxidant-induced cell and tissue injuries. In this study, a wheat (Triticum aestivum) HO1 gene TaHO1 was cloned and sequenced. It encodes a polypeptide of 31.7 kD with a putative N-terminal plastid transit peptide. The amino acid sequence of TaHO1 was found to be 78% similar to that of maize HO1. Phylogenetic analysis revealed that TaHO1 clusters together with the HO1-like sequences in plants. The purified recombinant TaHO1 protein expressed in Escherichia coli was active in the conversion of heme to biliverdin IXa (BV), and showed that the V-max was 8.8 U.mg(-1) protein with an apparent K-m value for hemin of 3.04 mu M. The optimum Tm and pH were 35 degrees C and 7.4, respectively. The result of subcellular localization of TaHO1 showed that the putative transit peptide was sufficient for green fluorescent protein (GFP) to localize in chloroplast and implied that TaHO1 gene product is at least localized in the chloroplast. Moreover, we found that TaHO1 mRNA could be differentially induced by the well-known nitric oxide (NO) donor sodium nitroprusside (SNP), gibberellin acid (GA), abscisic acid (ABA), hydrogen peroxide (H2O2) and NaCl treatments. Therefore, the results suggested that TaHO1 might play an important role in abiotic stress responses.

分类号：

  • 相关文献

[1]Isolation and characterization of a heme oxygenase-1 gene from Chinese cabbage. Jin, Qi-Jiang,Cui, Wei-Ti,Han, Bin,Feng, Jian-Fei,Xu, Sheng,Shen, Wen-Biao,Jin, Qi-Jiang,Cui, Wei-Ti,Han, Bin,Feng, Jian-Fei,Xu, Sheng,Shen, Wen-Biao,Yuan, Xing-Xing.

[2]Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.). Lv, Geng-Yin,Guo, Xiao-Guang,Xie, Li-Ping,Xie, Chang-Gen,Zhang, Xiao-Hong,Yang, Yuan,Xiao, Lei,Tang, Yu-Ying,Guo, Ai-Guang,Xu, Hong,Xie, Chang-Gen,Guo, Ai-Guang,Xu, Hong,Pan, Xing-Lai. 2017

[3]Heme Oxygenase-1 is Associated with Wheat Salinity Acclimation by Modulating Reactive Oxygen Species Homeostasis. Xie, Yanjie,Cui, Weiti,Shen, Wenbiao,Yang, Qing,Xie, Yanjie,Cui, Weiti,Shen, Wenbiao,Yang, Qing,Yuan, Xingxing. 2011

[4]Cloning and characterization of a heme oxygenase-2 gene from alfalfa (Medicago sativa L.). Fu, Guang-Qing,Jin, Qi-Jiang,Lin, Yu-Ting,Feng, Jian-Fei,Nie, Li,Shen, Wen-Biao,Fu, Guang-Qing,Jin, Qi-Jiang,Lin, Yu-Ting,Feng, Jian-Fei,Nie, Li,Shen, Wen-Biao,Zheng, Tian-Qing.

[5]Enhancement of Salinity Tolerance during Rice Seed Germination by Presoaking with Hemoglobin. Xu, Sheng,Hu, Bing,He, Ziyi,Ma, Fei,Feng, Jianfei,Shen, Wenbiao,Yang, Jie. 2011

[6]Induction of heme oxygenase-1 with beta-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa. Fu, Guangqing,Cui, Weiti,Wang, Yanqin,Shen, Wenbiao,Zhang, Liefeng,Ren, Yong,Zheng, Tianqing. 2011

[7]A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice. Chen, Hong,Wu, Han,Liu, Yanling,Zhou, Kunneng,Chen, Yilin,Liu, Yuqiang,Liu, Linglong,Jiang, Ling,Cheng, Zhijun,Ma, Xiaoding,Ma, Weiwei,Bi, Jingcui,Zhang, Xin,Guo, Xiuping,Wang, Jiulin,Lei, Cailin,Wu, Fuqing,Lin, Qibing. 2013

[8]A Novel Chloroplast-Localized Pentatricopeptide Repeat Protein Involved in Splicing Affects Chloroplast Development and Abiotic Stress Response in Rice. Tan, Junjie,Tan, Zhenhua,Sheng, Peike,Liu, Xuanming,Tan, Junjie,Tan, Zhenhua,Wu, Fuqing,Sheng, Peike,Heng, Yueqin,Ren, Yulong,Wang, Jiulin,Guo, Xiuping,Zhang, Xin,Cheng, Zhijun,Wang, Haiyang,Wan, Jianmin,Wang, Xinhua,Ren, Yulong,Jiang, Ling,Wan, Jianmin.

[9]Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses. Wang, Lijuan,Ma, Fei,Shen, Wenbiao,Xu, Sheng,Wang, Ren,Zheng, Tianqing,Chen, Huiping,Chen, Huiping.

[10]Enhancement of tolerance of Indian mustard (Brassica juncea) to mercury by carbon monoxide. Meng, De Kun,Yang, Zhi Min,Chen, Jian. 2011

[11]Non-noble metal catalyst for carbon monoxide selective oxidation in excess hydrogen. Jiang, Y,Xie, HM,Qiu, FL. 2005

[12]RNAi knockdown of rice SE5 gene is sensitive to the herbicide methyl viologen by the down-regulation of antioxidant defense. Xu, Sheng,Wang, Lijuan,Zhang, Bo,Han, Bin,Xie, Yanjie,Wang, Ning,Cui, Weiti,Shen, Wenbiao,Xu, Sheng,Wang, Lijuan,Zhang, Bo,Han, Bin,Xie, Yanjie,Wang, Ning,Cui, Weiti,Shen, Wenbiao,Xu, Sheng,Wang, Ren,Yang, Jie,Zhong, Weigong,Chen, Huiping.

[13]The role of carbon monoxide signaling in the responses of plants to abiotic stresses. He, Longfei,He, Huyi.

[14]Heme oxygenase 1 and abiotic stresses in plants. He, Longfei,He, Huyi.

[15]Heat Priming Induces Trans-generational Tolerance to High Temperature Stress in Wheat. Wang, Xiao,Xin, Caiyun,Cai, Jian,Zhou, Qin,Dai, Tingbo,Cao, Weixing,Jiang, Dong,Xin, Caiyun. 2016

[16]Distribution Characteristics of Soil Cadmium in Different Textured Paddy Soil Profiles and Its Relevance with Cadmium Uptake by Crops. Wang Zheng-yin,Qin Yu-sheng,Zhan Shao-jun,Yu Hua,Tu Shi-hua. 2013

[17]Molecular cloning, functional verification, and evolution of TmPm3, the powdery mildew resistance gene of Triticum monococcum L.. Zhao, C. Z.,Li, Y. H.,Dong, H. T.,Geng, M. M.,Liu, W. H.,Li, F.,Ni, Z. F.,Xie, C. J.,Sun, Q. X.,Zhao, C. Z.,Dong, H. T.,Geng, M. M.,Liu, W. H.,Li, F.,Ni, Z. F.,Xie, C. J.,Sun, Q. X.,Zhao, C. Z.,Wang, X. J.. 2016

[18]Wheat Optimized Fertilization of High Yield Field with Returning Whole Stalks into the Soil in Huang-huai-hai Plain. Sui, Xue-Yan,Wang, Meng,Wang, Yong,Guo, Hong-Hai,Li, Zhan,Zhang, Xiao-Dong. 2016

[19]Effects of Cadmium Stress on Alternative Oxidase and Photosystem II in Three Wheat Cultivars. Xu, Fei,Zhang, Zhong-Wei,Chen, Yang-Er,Wang, Xiao,Shang, Jing,Lin, Hong-Hui,Duan, Yong-Ping,Tu, Shi-Hua,Feng, Wen-Qiang. 2010

[20]Haynaldia villosa NAM-V1 is linked with the powdery mildew resistance gene Pm21 and contributes to increasing grain protein content in wheat. Zhao, Chuanzhi,Lv, Xindi,Li, Yinghui,Li, Feng,Geng, Miaomiao,Mi, Yangyang,Ni, Zhongfu,Xie, Chaojie,Sun, Qixin,Zhao, Chuanzhi,Lv, Xindi,Li, Yinghui,Li, Feng,Geng, Miaomiao,Mi, Yangyang,Ni, Zhongfu,Xie, Chaojie,Sun, Qixin,Zhao, Chuanzhi. 2016

作者其他论文 更多>>

微信小程序