Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV)

文献类型： 外文期刊

第一作者： Li, Bin

作者： Li, Bin;Ma, Junjie;Liu, Yanling;Mao, Li;Wen, Libin;Mao, Aihua;Zhang, Xuehan;Ni, Yanxiu;Guo, Rongli;Zhou, Junming;Yu, Zhengyu;Lv, Lixin;Wang, Xiaomin;He, Kongwang;Li, Bin;Ma, Junjie;Liu, Yanling;Mao, Li;Wen, Libin;Mao, Aihua;Zhang, Xuehan;Ni, Yanxiu;Guo, Rongli;Zhou, Junming;Yu, Zhengyu;Lv, Lixin;Wang, Xiaomin;He, Kongwang;Li, Bin;Ma, Junjie;Liu, Yanling;Mao, Li;Wen, Libin;Mao, Aihua;Zhang, Xuehan;Ni, Yanxiu;Guo, Rongli;Zhou, Junming;Yu, Zhengyu;Lv, Lixin;Wang, Xiaomin;He, Kongwang;Li, Bin;Xiao, Shaobo;Fang, Liurong;Chen, Huanchun

作者机构：

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2011 年 8 卷

页码：

收录情况： SCI

摘要： The recently discovered porcine boca-like virus (Pbo-likeV) is a member of the Parvoviridae family, genus Bocavirus, and it is potentially associated with swine disease. Several studies have associated Pbo-likeV with postweaning multisystemic wasting syndrome in pigs, but the full spectrum of clinical disease and the epidemiology of Pbo-likeV infection remain unclear. The availability of rapid and reliable molecular diagnostics would aid future studies of this novel virus. Thus, we developed a sensitive and specific TaqMan-based real-time PCR assay to target the Pbo-likeV NP1 gene. The assay reproducibly detected 20 copies of a recombinant DNA plasmid containing the NP1 gene, with a dynamic range of six orders of magnitude (10(2)-10(7) copies). The assay did not cross-react with other animal viruses. Clinical evaluation found that Pbo-likeV was present in Chinese swine herds at a frequency of 44.2% (114/258). Higher infection rates were found in diseased pigs (56.1%, 101/180) compared with healthy pigs (16.7%, 13/78) (P < 0.05). Our assay for the diagnosis and quantification of Pbo-likeV was highly sensitive and specific, and should provide a reliable real-time tool for epidemiological and pathogenetic study of Pbo-likeV infection.

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