Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid VP2 protein carrying immunoreactive epitope of porcine circovirus type 2

文献类型: 外文期刊

第一作者: Pan, Qunxing

作者: Pan, Qunxing;He, Kongwang;Pan, Qunxing;Huang, Kehe

作者机构:

关键词: virus-like particles;hybrid;PCV2;PPV

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN: 0264-410X

年卷期: 2008 年 26 卷 17 期

页码:

收录情况: SCI

摘要: Virus-Like particles (VLPs) are non-repticative vectors for delivery of heterologous epitopes and induction of immune responses. In this study, a self-assembled porcine parvovirus (PPV) VP2 capsid protein [PPV:VLP-(PCV2)] carrying immunoreactive epitopes, residues 165-200 from the Porcine circovirus 2 (PCV2) virus nucleoprotein was constructed. Immunogenicity study was carried out with hybrid VLPs derived from HEK-293 cells infected with recombinant adenovirus vectors. To our knowledge, this study presents the first demonstration that hybrid non-repticative PPV VLPs carrying PCV2 immunoreactive epitopes can induce stronger antibody responses against PCV2 than recombinant adenovirus of PCV2 ORF2, in the absence of any adjuvant. The hybrid VLPs [PPV:VLP-(PCV2)] might be a promising candidate vaccine for better prevention of the diseases associated with PCV2 as well as with co-infection by PCV2 and PPV. (C) 2008 Elsevier Ltd. All rights reserved.

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