Allicin enhances urea-N conversion to microbial-N by inhibiting urease activity and modulating the rumen microbiome in cattle

文献类型： 外文期刊

第一作者： Zhang, Shiqi

作者： Zhang, Shiqi;Zheng, Nan;Zhao, Shengguo;Wang, Jiaqi

作者机构：

关键词： Allicin; Rumen microbiome; Urease inhibition; Urea nitrogen; Microbial nitrogen

期刊名称：MICROBIOME （ 影响因子：12.7； 五年影响因子：16.6 ）

ISSN： 2049-2618

年卷期： 2025 年 13 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundUrea serves as a vital nonprotein nitrogen source in ruminant nutrition, but its efficient utilization is often hampered due to rapid urease activity in the rumen. This study explores the potential of allicin, a garlic-derived compound, as a urease inhibitor to improve urea nitrogen utilization. Enzyme inhibition kinetics and molecular docking were used to identify allicin's interaction sites on urease. Additionally, metagenomic and 15N-urea metabolic flux analyses were conducted to evaluate allicin's impact on microbial populations and urea-N metabolism.ResultsAllicin was identified as an inhibitor of ruminal urease, with an IC50 of 126.77 +/- 1.21 mu M. Molecular docking studies have shown that allicin forms hydrogen bonds with key urease residues, leading to the preemption of the urease active site and thus impeding urea binding. In a simulated rumen environment, allicin significantly reduced urea hydrolysis and ammonia production. Furthermore, allicin modified the rumen microbial community by inhibiting Prevotella species while promoting the growth of Ruminobacter species and Denitrobacterium detoxificans. A 15N-urea metabolic flux analysis revealed that allicin facilitated the incorporation of urea-derived nitrogen into microbial amino acids and nucleotides.ConclusionAllicin effectively inhibits urease activity in the rumen, enhancing the conversion of urea-N into microbial biomass. These findings suggest that allicin has significant potential to optimize urea metabolism in the rumen, offering a novel strategy for improving ruminant nitrogen nutrition.ALEcB6nmbxpuaJXpdrTmMrVideo AbstractConclusionAllicin effectively inhibits urease activity in the rumen, enhancing the conversion of urea-N into microbial biomass. These findings suggest that allicin has significant potential to optimize urea metabolism in the rumen, offering a novel strategy for improving ruminant nitrogen nutrition.ALEcB6nmbxpuaJXpdrTmMrVideo Abstract

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序