Molecular characterization of a wheat - Thinopyrum ponticum partial amphiploid and its derivatives for resistance to leaf rust

文献类型： 外文期刊

第一作者： Li, HJ

作者： Li, HJ;Chen, O;Conner, RL;Guo, BH;Zhang, YM;Graf, RJ;Laroche, A;Jia, X;Liu, GS;Chu, CC

作者机构：

关键词： Lr24;genomic in situ hybridization;sequence-tagged site;random amplified polymorphic DNA

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

ISSN： 0831-2796

年卷期： 2003 年 46 卷 5 期

页码：

收录情况： SCI

摘要： Leaf rust (caused by Puccinia triticina Eriks.) occurs annually in most wheat-growing areas of the world. Thinopyrum ponticum (Podp.) Z.-W. Liu & R.-C. Wang has provided several leaf rust resistance genes to protect wheat from this fungal disease. Three chromosome substitution lines, Ji806, Ji807, and Ji859, and two chromosome addition lines, Ji791 and Ji924, with a winter growing habit were developed from crosses between wheat (Triticum aestivum L. ern Thell.) and the wheat - Th. ponticum partial amphiploid line 693. These lines were resistant to leaf rust isolates from China. Sequence-tagged site (STS) analysis with the J09-STS marker, which is linked to the gene Lr24, revealed that the partial amphiploid line 693 and all of the substitution and addition lines carried gene Lr24. Genomic in situ hybridization (GISH) analysis was carried out on chromosome preparations using total genomic DNA from Pseudoroegneria strigosa (M. Bieb) A. Love (St genome, 2n = 14) as a probe in the presence of total genomic DNA from T aestivum 'Chinese Spring' wheat (ABD genomes, 2n = 42). The GISH analysis demonstrated that these lines had a pair of chromosomes displaying the typical pattern of a J(s) genome chromosome. This indicates that the chromosome that carries gene Lr24 belonged to the Js genome of Th. ponticum. In addition to 40 wheat chromosomes, eight J(s) and eight J genome chromosomes were also differentiated by GISH in the partial amphiploid line 693. Since most sources of Lr24 have a red grain color, the white-colored seeds in all of these substitution and addition lines, together with high protein content in some of the lines, make them very useful as a donor source for winter wheat breeding programs.

分类号：

  • 相关文献

[1]Identification of wheat-Thinopyrum intermedium 2Ai-2 ditelosomic addition and substitution lines with resistance to barley yellow dwarf virus. Lin, ZS,Huang, DH,Du, LP,Ye, XG,Xin, ZY. 2005

[2]Phenotypic and Genotypic Characterization of Phytophthora infestans Isolates from China. Li, Benjin,Chen, Qinghe,Lv, Xin,Lan, Chengzhong,Zhao, Jian,Qiu, Rongzhou,Weng, Qiyong.

[3]Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb. Li, J.,Liu, X. H.,Zhang, Y. S.,Wen, S. S.. 2014

[4]Genetic diversity among populations and breeding lines from recurrent selection in Brassica napus as revealed by RAPD markers. Yuan, M,Zhou, Y,Liu, D. 2004

[5]Distribution, serological and molecular characterization of Vibrio parahaemolyticus from shellfish in the eastern coast of China. Zhao, Feng,Zhou, De-qing,Cao, Hui-hui,Ma, Li-ping,Jiang, Yan-hua,Zhao, Feng. 2011

[6]Genetic variation within Chinese and western cultivated oat accessions. Baohong, G,Zhou, X,Murphy, JP. 2003

[7]A genetic diversity analysis of wild Lespedeza populations based on morphological characters, allozyme and RAPD methods. Zhang, J. Y.,Yuan, Q. H.,Meng, Y. Q.,Li, X. L.,Nan, Z. B.,Wang, Y. R.,Zhang, W. S.. 2007

[8]Genetic Structure and Aggressiveness of Rhizoctonia solani AG1-IA, the Cause of Sheath Blight of Rice in Southern China. Wang, Ling,Liu, Lian Meng,Wang, Zhi Gang,Huang, Shi Wen. 2013

[9]Analysis of genetic diversity of salt-tolerant alfalfa germplasms. Jiang, J.,Yang, B. L.,Wu, Y. N.,Jin, H.,Xia, T.,Yu, S. M.,Li, J. R.. 2015

[10]Development of Head Smut Resistance-linked Sequence Characterized Amplified Regions Markers in Sorghum. Li, Yueying,Ma, Lianju,Ma, Chunyan,Li, Xuemei,Lu, Dan,Lu, Shuiyi,Hao, Lin,Zou, Jianqiu. 2012

[11]Genetic diversity in Colletotrichum gloeosporioides from Stylosanthes spp. at centers of origin and utilization. Weeds, PL,Chakraborty, S,Fernandes, CD,Charchar, MJD,Ramesh, CR,Kexian, Y,Kelemu, S. 2003

[12]The genetic analysis and germplasm identification of the gametophytes of Undaria pinnatifida (Phaeophyceae) with RAPD method. Wang, Di,Wang, Xiuliang,Li, Dapeng,Wang, Feijiu,Duan, Delin.

[13]Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers. Wang, Xiaoxiao,Wang, Yingying,Wang, Chen,Chen, Yu,Chen, Yu,Feng, Shouli,Zhao, Ting,Zhou, Baoliang,Chen, Yu. 2016

[14]Inducement and identification of chromosome introgression and translocation of Gossypium australe on Gossypium hirsutum. Wang, Yingying,Feng, Shouli,Li, Sai,Tang, Dong,Chen, Yu,Chen, Yu,Zhou, Baoliang,Chen, Yu,Chen, Yu. 2018

[15]Molecular cytogenetic characterization of two partial wheat Elytrigia elongata amphiploids resistant to powdery mildew. He, Fang,Xu, Jinqiu,Qi, Xiaolei,Bao, Yinguang,Li, Xingfeng,Wang, Honggang,Zhao, Fengtao.

[16]A novel genome of C and the first autotetraploid species in the Setaria genus identified by genomic in situ hybridization. Wang, Yongqiang,Zhi, Hui,Li, Wei,Li, Haiquan,Wang, Yongfang,Diao, Xianmin,Wang, Yongfang,Huang, Zhanjing,Diao, Xianmin,Zhi, Hui,Diao, Xianmin.

[17]Microdissection of Haynaldia villosa Telosome 6VS and Cloning of Species-specific DNA Sequences. Kong, FJ,Chen, X,Ma, YZ,Xin, ZY,Li, LC,Zhang, ZY,Lin, ZS.

[18]Cytogenetic and molecular identification of small-segment chromosome translocation lines from wheat-rye substitution lines to create wheat germplasm with beneficial traits. Song, Wei-Fu,Zhang, Xiao-Mei,Li, Ji-Lin,Ding, Hai-Yan,Xiao, Zhi-Min,Xin, Wen-Li,Song, Qing-Jie,Zhao, Hai-Bin,Zhang, Yan-Bin,Zhang, Chun-Li.

[19]Induction and transmission of wheat-Haynaldia villosa chromosomal translocations. Cao, Yaping,Bie, Tongde,Wang, Xiue,Chen, Peidu,Cao, Yaping,Bie, Tongde. 2009

[20]Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp trichophorum. Yang, ZJ,Li, GR,Chang, ZJ,Zhou, JP,Ren, ZL. 2006

作者其他论文 更多>>

微信小程序