Vacuolar MATE/DTX protein-mediated cucurbitacin C transport is co-regulated with bitterness biosynthesis in cucumber

文献类型： 外文期刊

第一作者： Ma, Yongshuo

作者： Ma, Yongshuo;Li, Dawei;Zhong, Yang;Lucas, William J.;Huang, Sanwen;Ma, Yongshuo;Wang, Xiaohan;Li, Legong;Osbourn, Anne;Lucas, William J.;Shang, Yi;Shang, Yi

作者机构：

关键词： cucurbitacin; gene cluster; MATE; transporter; triterpene

期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）

ISSN： 0028-646X

年卷期： 2023 年 238 卷 3 期

页码：

收录情况： SCI

摘要： Membrane-localized transporters constitute important components for specialized metabolism in plants. However, due to the vast array of specialized metabolites produced by plants, and the large families of transporter genes, knowledge about the intracellular and intercellular transport of plant metabolites is still in its infancy. Cucurbitacins are bitter and defensive triterpenoids produced mainly in the cucurbits. Using a comparative genomics and multi-omics approach, a MATE gene (CsMATE1), physically clustered with cucurbitacin C (CuC) biosynthetic genes, was identified and functionally shown to sequester CuC in cucumber leaf mesophyll cells. Notably, the CuC transport process is strictly co-regulated with CuC biosynthesis. CsMATE1 clustering with bitterness biosynthesis genes may provide benefits and a basis for this feedback regulation on CuC sequestration and biosynthesis. Identification of transport systems for plant-specialized metabolites can accelerate the metabolic engineering of high-value-added compounds by simplifying their purification process.

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