Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

文献类型: 外文期刊

第一作者: Liu Li-ying

作者: Liu Li-ying;Duan Liu-sheng;Zhang Jia-chang;Zhang Xiao-lan;Zhang Zhen-xian;Ren Hua-zhong;Mi Guo-quan

作者机构: China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China;Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China

关键词: ABA;low-temperature stress;LOS5;transgenic plant;cucumber;salt stress

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 2020影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2013 年 12 卷 5 期

页码:

收录情况: SCI

摘要: Low temperature and high salinity are the major abiotic stresses that restrict cucumber growth and production, breeding materials with multiple abiotic resistance are in greatly need. Here we investigated the effect of introducing the LOSS gene, a key regulator of ABA biosynthesis in Arabidopsis thaliana, under the stress-responsive RD29A promoter into cucumber (Cucumis sativus L. cv. S516). We found that T-1 RD29A-LOS5 transgenic lines have enhanced tolerance to cold and salt stresses. Specifically, transgenic lines exhibited dwarf phenotypes with reduced leaf number, shorter internode, decreased length of the biggest leaf, fewer female flowers, shorter fruit neck and lower vitamin C (Vc). The increased cold tolerance can be reflected from the significantly decreased cold index, the reduced electrolyte leakage index and the MDA content upon cold treatment as compared to those in the control. This may result from the accumulation of internal ABA, soluble sugars and proline, and the enhanced activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the transgenic lines. Under salt treatment, the transgenic lines exhibited increased germination index, vigor index, more lateral roots and increased root fresh weight. Moreover, RD29A-LOS5 transgenic plants displayed quicker responses in salt stress than that in low-temperature stress.

分类号:

  • 相关文献

[1]Exogenous nitric oxide delays salt-induced leaf senescence in cotton (Gossypium hirsutum L.). Kong, Xiangqiang,Wang, Tao,Li, Weijiang,Tang, Wei,Zhang, Dongmei,Dong, Hezhong,Wang, Tao,Dong, Hezhong.

[2]Soaking in H2O2 regulates ABA biosynthesis and GA catabolism in germinating cotton seeds under salt stress. Kong, Xiangqiang,Luo, Zhen,Zhang, Yanjun,Li, Weijiang,Dong, Hezhong.

[3]Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice. Chen, Yixing,Zhou, Xiaojin,Chang, Shu,Chu, Zhilin,Wang, Hanmeng,Han, Shengcheng,Wang, Yingdian,Zhou, Xiaojin.

[4]Homologous HAP5 subunit from Picea wilsonii improved tolerance to salt and decreased sensitivity to ABA in transformed Arabidopsis. Li, Lingli,Wei, Jing,Huang, Guixue,Zhang, Dun,Liu, Yong,Zhang, Lingyun,Yu, Yanli.

[5]MicroRNA and target gene responses to salt stress in grafted cucumber seedlings. Li, Yansu,Li, Chaohan,Bai, Longqiang,He, Chaoxing,Yu, Xianchang,Li, Chaohan. 2016

[6]Growth arrest specific gene 2 in tilapia (Oreochromis niloticus): molecular characterization and functional analysis under low-temperature stress. Yang, ChangGeng,Wu, Fan,Lu, Xing,Jiang, Ming,Liu, Wei,Yu, Lijuan,Tian, Juan,Wen, Hua. 2017

[7]An iNTT system for the large-scale screening of differentially expressed, nuclear-targeted proteins: cold-treatment-induced nucleoproteins in Rye (Secale cereale L.). Cao, Xinyou,Liu, Yangna,Xu, Zhaoshi,Li, Liancheng,Zhou, Yongbin,Chen, Ming,Ma, Youzhi,Cao, Xinyou,Liu, Yangna,Xu, Zhaoshi,Li, Liancheng,Zhou, Yongbin,Chen, Ming,Ma, Youzhi,Cao, Xinyou,Chen, Xueyan,Liu, Jianjun,Zhao, Zhendong,Cao, Xinyou,Chen, Xueyan,Liu, Jianjun,Zhao, Zhendong,Cao, Xinyou,Chen, Xueyan,Liu, Jianjun,Zhao, Zhendong,Chen, Xueyan. 2016

[8]Analysis of differential gene expression under low-temperature stress in Nile tilapia (Oreochromis niloticus) using digital gene expression. Yang, Changgeng,Jiang, Ming,Wen, Hua,Tian, Juan,Liu, Wei,Wu, Fan,Gou, Gengwu.

[9]Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. ZhongQun, He,ChaoXing, He,ZhiBin, Zhang,ZhiRong, Zou,HuaiSong, Wang.

[10]Over-expression of the Gr5(aroA) gene from glyphosate-contaminated soil confers high tolerance to glyphosate in tobacco. Wang, Jin,Lu, Wei,Zhu, Yu,Lin, Min,Zuo, Kaijing,Tang, Kexuan,Ye, Chun. 2014

[11]Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase. Wang, Xiaoxue,Wu, Ningfeng,Guo, Jun,Chu, Xiaoyu,Tian, Jian,Fan, Yunliu,Yao, Bin.

[12]PHYSIOLOGICAL CHARACTERIZATION OF TRANSGENIC ALFALFA (MEDICAGO SATIVA) PLANTS FOR IMPROVED DROUGHT TOLERANCE. Jiang, Qingzhen,Zhang, Ji-Yi,Guo, Xiulin,Monteros, Maria J.,Wang, Zeng-Yu,Guo, Xiulin.

[13]Over-expression of ApKUP3 enhances potassium nutrition and drought tolerance in transgenic rice. Song, Z. -Z.,Song, Z. -Z.,Yang, S. -Y.,Su, Y. -H.,Yang, S. -Y.,Zuo, J.,Zuo, J..

[14]Recent Advances in Utilizing Transcription Factors to Improve Plant Abiotic Stress Tolerance by Transgenic Technology. Wang, Hongyan,Wang, Honglei,Shao, Hongbo,Shao, Hongbo,Tang, Xiaoli. 2016

[15]Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants. Yang YanMei,Fang RongXiang,Chen XiaoYing,Yang YanMei,Fang RongXiang,Chen XiaoYing,Yang YanMei,Li Xia,Yang Hui,Qian Yuan,Zhang You. 2011

[16]Over-expression of Oryza sativa Xrn4 confers plant resistance to virus infection. Jiang, Shanshan,Jiang, Liangliang,Jiang, Shanshan,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei. 2018

[17]Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease. Kelemu, S,Jiang, CS,Huang, GX,Segura, G. 2005

[18]Detection of unintended effects in genetically modified herbicide-tolerant (GMHT) rice in comparison with non-target phenotypic characteristics. Xiao, Guoying,Jiang, Xianbin,Jiang, Xianbin. 2010

[19]Expressing p20 hairpin RNA of Citrus tristeza virus confers Citrus aurantium with tolerance/resistance against stem pitting and seedling yellow CTV strains. Bei Xue-jun,Zhang Yong-yan,Lu Zhi-ming,Cheng Chun-zhen,Bei Xue-jun,Zhang Yong-yan,Zhong Guang-yan,Yang Jia-wei,Yan Hu-bin,Cheng Chun-zhen,Zhong Guang-yan. 2015

[20]Effects of Bt transgenic cotton lines on the cotton bollworm parasitoid Microplitis mediator in the laboratory. Liu, XX,Zhang, QW,Zhao, JZ,Li, HC,Xu, BL,Ma, XM. 2005

作者其他论文 更多>>