Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L.

文献类型： 外文期刊

第一作者： Cao Ya-cong

作者： Cao Ya-cong;Sul Xiao-lei;Zhang Zhen-xian;Cao Ya-cong;Zhang Zheng-hai;Wang Li-hao;Zhang Bao-xi

作者机构：

关键词： Capsicum annuum L.;fruit;GID1;DELLA

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 4 期

页码：

收录情况： SCI

摘要： Fruit set and development are affected by many phytohormones, including gibberellin. Little is known regarding molecular mechanism underlying gibberellin mediated fruit set and development especially in Capsicum. Three gibberellin receptors, CaGID1b.1, CaGID1b.2 and CaGID1c, and a DELLA protein, CaGAI, have been identified in Capsicum annuum L. During the fruit development, the expression level of CaGID1c was low, and the expression fold change is mild. However, CaGID1b.1 and CaGID1b.2 were relatively higher and more acute, which indicates that CaGID1b.1 and CaGID1b.2 may play an important role in fruit pericarp, placenta and seed. Ectopic expressions of CaGID1b.1, CaGID1b.2 and CaGID1c in Arabidopsis double mutant gid1c gid1c increased plant height, among which CaGID1b.2 had the most significant effect; CaGAI reduced plant height in double mutant rga-24/gai-t6, having a similar function to AtGID1 and AtGAI in stem elongation. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that CaGID1b.1 and CaGID1b.2 interact with CaGAI in a GA-dependent manner, while CaGID1c interacts with CaGAI in a GA-independent manner. Our study reveals the key elements during gibberellin signaling in Capsicum and supports the critical importance of gibberellin for Capsicum fruit set and development.

分类号：

  • 相关文献

[1]Cloning and expression analysis of four DELLA genes in peanut. An, J.,Zhao, Y. X.,Wang, X. J.,An, J.,Hou, L.,Li, C.,Wang, C. X.,Xia, H.,Zhao, C. Z.,Li, C. S.,Wang, X. J.,Li, C.,Zheng, Y. X..

[2]Characterization of seed fatty acid accumulation in DELLA mutant lines of Arabidopsis. Li, Zhilan,Jiang, Yuxiao,Jiang, Chiyu,Zhou, Longhua,Jiang, Lixi,Hua, Shuijin,Chen, Xiaoyang,Ren, Yun.

[3]Cloning and expression analysis of CaPIP1-1 gene in pepper (Capsicum annuum L.). Yin, Yan-Xu,Zhang, Huai-Xia,Xiao, Huai-Juan,Jin, Jing-Hao,Ji, Jiao-Jiao,Jing, Hua,Chen, Ru-Gang,Arisha, Mohamed Hamed,Gong, Zhen-Hui,Wang, Shu-Bin,Arisha, Mohamed Hamed.

[4]Comparative proteome analysis in hot pepper (Capsicum annuum L.) after space flight. Xie, L. B.,Wang, X.,Zhou, Y.,Chen, L. X.,Gao, Y. L.,Guo, Y. H.,Peng, M.,Liu, L. X.. 2017

[5]Cloning and characterization of the CarbcL gene related to chlorophyll in pepper (Capsicum annuum L.) under fruit shade stress. Wang, Shu-Bin,Pan, Bao-Gui,Diao, Wei-Ping,Tian, Shi-Lin,Shah, Syed N. M.,Gong, Zhen-Hui,Tian, Shi-Lin,Shah, Syed N. M.. 2015

[6]Characterization and expression profile of CaNAC2 pepper gene. Guo, Wei-Li,Chen, Ru-Gang,Yin, Yan-Xu,Gong, Zhen-Hui,Guo, Wei-Li,Chen, Bi-Hua,Du, Xiao-Hua,Zhang, Yu-Yuan,Wang, Shu-Bin. 2015

[7]Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility. Dai, X. Z.,Zhang, Z. Q.,Zou, X. X.,Deng, M. H.,Zhu, H. S.,Zhou, H.,Wen, J. F.,Huo, J. L.. 2012

[8]Development of pepper SSR markers from sequence databases. Huang, SW,Zhang, BX,Milbourne, D,Cardle, L,Yang, GM,Guo, JZ. 2001

[9]Overexpression of the CaTIP1-1 Pepper Gene in Tobacco Enhances Resistance to Osmotic Stresses. Yin, Yan-Xu,Xiao, Huai-Juan,Zhang, Huai-Xia,Zhang, Zhen,Jing, Hua,Zhang, Ying-Li,Chen, Ru-Gang,Gong, Zhen-Hui,Wang, Shu-Bin,Zhang, Ying-Li. 2014

[10]Effect of continuous negative pressure water supply on the growth, development and physiological mechanism of Capsicum annuum L.. Li Di,Shao Hong-ying,Wang Peng,Long Huai-yu,Zhang Shu-xiang,Wu Xue-ping. 2017

[11]Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress. Guo, Meng,Liu, Jin-Hong,Lu, Jin-Ping,Zhai, Yu-Fei,Wang, Hu,Gong, Zhen-Hui,Lu, Ming-Hui,Wang, Shu-Bin. 2015

[12]Molecular characteristics and expression of cytoplasmic genes in cytoplasmic male sterility of pepper (Capsicum annuum L.). Zhang Zhuqing,Dai Xiongze,Zou Xuexiao,Ou Lijun,Ou Lijun. 2012

[13]Cloning and functional analyses of pepper CaRKNR involved in Meloidogyne incognita resistance. Mao, Zhenchuan,Zhu, Pingping,Liu, Feng,Huang, Yonghong,Ling, Jian,Chen, Guohua,Yang, Yuhong,Feng, Dongxin,Xie, Bingyan.

[14]RELATIONSHIP OF ASCORBIC ACID METABOLISM WITH THE CYTOPLASMIC MALE STERILITY IN PEPPER (CAPSICUM ANNUUM L.). Deng, Minghua,Deng, Minghua,Huo, Jinlong,Zhu, Haishan,Lv, Junheng,Dai, Xiongze,Zhang, Zhuqing,Zou, Xuexiao. 2015

[15]A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.). Wang, L. H.,Gu, X. H.,Hua, M. Y.,Mao, S. L.,Zhang, Z. H.,Zhang, B. X.,Gu, X. H.,Hua, M. Y.,Yun, X. F.,Peng, D. L..

[16]Development of a large number of SSR and InDel markers and construction of a high-density genetic map based on a RIL population of pepper (Capsicum annuum L.). Zhang, Xiao-fen,Wang, Qian,Zhang, Xiao-fen,Sun, Hong-he,Xu, Yong,Chen, Bin,Yu, Shuan-cang,Geng, San-sheng.

[17]Apple MdACS6 Regulates Ethylene Biosynthesis During Fruit Development Involving Ethylene-Responsive Factor. Li, Tong,Tan, Dongmei,Jiang, Zhongyu,Wei, Yun,Zhang, Lichao,Li, Xinyue,Yuan, Hui,Wang, Aide,Liu, Zhi.

[18]Fruit protected cultivation in China. Gao, H,Wang, S,Wang, J.

[19]Determining hydrogen cyanamide in fruit by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and HPLC with fluorescence detection. Cai, Xiao-Ming,Zhao, Hua,Wu, Min,Hu, Xiu-Qing,He, Hong-Mei,Zhang, Chang-Peng,Zhang, Chun-Rong,Li, Zhen,Cai, Xiao-Ming,Zhao, Hua,Wu, Min,Hu, Xiu-Qing,He, Hong-Mei,Zhang, Chang-Peng,Zhang, Chun-Rong,Li, Zhen,Cai, Xiao-Ming,Zhao, Hua,Wu, Min,Hu, Xiu-Qing,He, Hong-Mei,Zhang, Chang-Peng,Zhang, Chun-Rong,Li, Zhen.

[20]Rapid Analysis of Tetraconazole Residues in Fruits and Vegetables using Ethyl Acetate Extraction and Gas Chromatography-tandem Mass Spectrometry. Doug, Fengshou,Liu, Xingang,Li, Jing,Li, Yuanbo,Zheng, Yongquan,Shan, Weili.

作者其他论文 更多>>

微信小程序