Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2

文献类型: 外文期刊

第一作者: Jin, Qianyue

作者: Jin, Qianyue;Wang, Yinbiao;Chen, Wen;Jin, Qianyue;Yang, Jifei;Lu, Qingxia;Guo, Junqing;Deng, Ruiguang;Wang, Yinbiao;Wang, Shihong;Wang, Shufen;Chen, Wen;Zhi, Yubao;Wang, Li;Yang, Suzhen;Zhang, Gaiping;Wang, Shufen;Wang, Shufen

作者机构:

关键词: Enzyme-linked immunosorbent assay;immunochromatographic strip;Porcine circovirus-2

期刊名称:JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION ( 影响因子:1.279; 五年影响因子:1.522 )

ISSN: 1040-6387

年卷期: 2012 年 24 卷 6 期

页码:

收录情况: SCI

摘要: A rapid (<5 min) immunochromatographic strip using a colloidal gold-labeled antigen probe was successfully developed and applied for the detection of Porcine circovirus-2 (PCV-2) antibodies in swine. Recombinant Cap protein truncated nuclear localization signal of PCV-2, was expressed and labeled with colloidal gold. This conjugate was dispensed on a conjugate pad as the detector. Staphylococcal protein A and purified porcine anti-PCV-2 antibodies were blotted on a nitrocellulose membrane for the test and control lines, respectively. Sensitivity and specificity of this strip test was evaluated using PCV-2 antisera as well as other sera from pigs infected with a variety of swine viruses. For the validation of this strip test, 500 clinical swine serum samples were assessed both by the strip and a commercial enzyme-linked immunosorbent assay (ELISA) kit. The agreement between the immunochromatographic strip and ELISA kit was 94.00%. This strip possesses high sensitivity and specificity and may be useful as a candidate for rapid diagnosis of PCV-2 antibodies in the field.

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