Regulation of fertilization in male rats by CatSper2 knockdown

文献类型： 外文期刊

第一作者： Zhang, Zhen

作者： Zhang, Zhen;Wang, Gen-Lin;Li, Hui-Xia;Li, Lian;Cui, Qun-Wei;Wei, Cheng-Bin;Zhou, Fei

作者机构：

关键词： Catsper2;in vivo electroporation;male contraception;rete testis microinjection

期刊名称：ASIAN JOURNAL OF ANDROLOGY （ 影响因子：3.285； 五年影响因子：4.237 ）

ISSN： 1008-682X

年卷期： 2012 年 14 卷 2 期

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收录情况： SCI

摘要： Interest in ion channels as drug targets for contraception has grown with the realization that certain ion channel subunits are located exclusively in sperm. Selective knockdown of ion channel subunits can lead to infertility without ill effects, and selective inhibitors and/or openers of these ion channels could interfere with sperm function. In this study, in vivo electroporation (EP) and rete testis microinjection-mediated plasmid DNA were adopted to silence CatSper2 expression, which is essential in sperm hyperactivation. The results showed that high transfection efficiency and expression were achieved by plasmid DNA that was directly injected into the rete testis. As a result of the expression of CatSper2 being blocked, the treatment group showed significantly lower (P<0.05) hyperactivation rate, fertilization rate in vitro, migration motility in viscoelastic solution and intracellular Ca2+ peak. The low hyperactivation and fertilization rates lasted for 60 days. Meanwhile, analysis of the sperm survival rate and testis histology indicated that in vivo EP had no significant effect on the function of the testis, spermatogenesis or sperm activity. The present study demonstrated that it was feasible to achieve male contraception by silencing the expression of CatSper2, the key protein involved in sperm hyperactivation. Asian Journal of Andrology (2012) 14, 301-309; doi:10.1038/aja.2011.118; published online 17 October 2011

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