Endoribonuclease activities of porcine reproductive and respiratory syndrome virus nsp11 was essential for nsp11 to inhibit IFN-beta induction

文献类型： 外文期刊

第一作者： Shi, Xibao

作者： Shi, Xibao;Wang, Li;Li, Xuewu;Zhang, Gaiping;Guo, Junqing;Zhao, Dong;Chai, Shujun;Deng, Ruiguang

作者机构：

关键词： Porcine reproductive and respiratory syndrome virus (PRRSV);Interferon beta;nsp11;Endoribonuclease;IFN-regulatory factor 3

期刊名称：MOLECULAR IMMUNOLOGY （ 影响因子：4.407； 五年影响因子：4.227 ）

ISSN： 0161-5890

年卷期： 2011 年 48 卷 12-13 期

页码：

收录情况： SCI

摘要： Previous studies have shown that PRRSV nsp11 which was an endoribonuclease, was an interferon antagonist, however, the mechanism that nsp11 inhibited IFN-beta production was unclear. To explore whether the endoribonuclease was required for nsp11 to disrupt the IFN-beta production, substitutions of the presumed catalytic histidine and lysine residues of nsp11 were introduced into plasmid pcDNA 3.1-FLAG. The results showed that mutation that inactivated endoribonuclease made nsp11 lose its ability to inhibit Poly(I:C)-induced IFN-beta promoter activity. In conclusion, our present work indicated that the endoribonuclease activity of nsp11 was essential for nsp11 to inhibit the IFN-beta induction. (C) 2011 Elsevier Ltd. All rights reserved.

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