Identification of the streptothricin and tunicamycin biosynthetic gene clusters by genome mining in Streptomyces sp strain fd1-xmd

文献类型： 外文期刊

第一作者： Yu, Yucong

作者： Yu, Yucong;Tang, Biao;Dai, Ruixue;Zhang, Bo;Chen, Liqiang;Zhao, Guoping;Ding, Xiaoming;Tang, Biao;Yang, Hua;Zhao, Guoping

作者机构：

关键词： Antibiotics;Complete genome;Heterologous expression;Streptomyces sp strain fd1-xmd;Synthetic biology

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2018 年 102 卷 6 期

页码：

收录情况： SCI

摘要： The genus Streptomyces have been highly regarded for their important source of natural products. Combined with the technology of genome sequencing and mining, we could identify the active ingredients from fermentation broth quickly. Here, we report on Streptomyces sp. strain fd1-xmd, which was isolated from a soil sample collected in Shanghai. Interestingly, the fermentation broth derived from this strain demonstrated broad-spectrum antimicrobial activity against gram-positive bacteria, gram-negative bacteria, and eukaryotes. To identify the antimicrobial substances and their biosynthetic gene clusters, we sequenced the fd1-xmd strain and obtained a genome 7,929,999 bp in length. The average GC content of the chromosome was 72.5 mol%. Knockout experiments demonstrated that out of eight biosynthetic gene clusters we could identify, two are responsible for the biosynthesis of the antibiotics streptothricin (ST) and tunicamycin (TM). The ST biosynthetic gene cluster from fd1-xmd was verified via successful heterologous expression in Streptomyces coelicolor M1146. ST production had a yield of up to 0.5 g/L after the optimization of culture conditions. This study describes a novel producer of ST and TM and outlines the complete process undertaken for Streptomyces sp. strain fd1-xmd genome mining.

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