Mining and expression analysis of candidate genes involved in regulating the chilling requirement fulfillment of Paeonia lactiflora 'Hang Baishao'

文献类型： 外文期刊

第一作者： Zhang, Jiaping

作者： Zhang, Jiaping;Li, Danqing;Zhang, Dong;Zhang, Jiao;Xia, Yiping;Shi, Xiaohua;Zhou, Jianghua;Zhu, Kaiyuan;Qiu, Shuai;Wei, Jianfen

作者机构：

关键词： Chilling requirement;Bud dormancy;Gene mining;Temperature response;Photoperiod response;SUPPRESSPOR OF OVEREXPRESSION OF CONSTANS1 (SOC1);Herbaceous peony (Paeonia lactiflora)

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2017 年 17 卷

页码：

收录情况： SCI

摘要： Background: The artificial enlargement of the planting area and ecological amplitude of ornamentals for horticultural and landscape applications are significant. Herbaceous peony (Paeonia lactiflora Pall.) is a world-famous ornamental with attractive and fragrant flowers and is mainly planted in temperate and cool areas. Comparatively higher winter temperatures in the subtropical and tropical Northern Hemisphere result in a deficit of chilling accumulation for bud dormancy release, which severely hinders "The southward plantation of herbaceous peony". Studies on the dormancy, chilling requirement (CR) and relevant molecular mechanisms of peony are needed to enhance our ability to extend the range of this valuable horticultural species. Results: Based on natural and artificial chilling experiments, and chilling hour (CH) and chilling unit (CU) evaluation systems, the lowest CR of 'Hang Baishao' was between 504.00 and 672.00 CHs and the optimal CR was 672.00 CHs and 856.08 CUs for achieving strong sprouting, growth and flowering performance. Transcriptome sequencing and gene identification by RNA-Seq were performed on 'Hang Baishao' buds during the dormancy and sprouting periods. Six gene libraries were constructed, and 66 temperature-and photoperiod-associated unigenes were identified as the potential candidate genes that may regulate or possibly determine CR characteristics. The difference in the expression patterns of SUPPRESSPOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) between the winters of 2012-2013 and 2015-2016, and the difference of CR fulfillment periods also between these two winters represented the interesting congruent relationships. This correlation was also observed for WRKY DNA-BINDING PROTEIN 33 (WRKY 33). Conclusions: Combined with the results acquired from all of experiments, 'Hang Baishao' was confirmed to be a superb peony resource that have significantly low CR characteristics. The two genes of SOC1 and WRKY33 are likely involved in determining the CR amount and fulfillment period of ' Hang Baishao'. HEAT SHOCK PROTEIN, OSMOTIN and TIMING OF CAB EXPRESSION 1 also deserve attention for the CR research. This study could contribute to the knowledge of the deep factors and mechanisms that regulate CR characteristics, and may be beneficial for breeding new germplasms that have low CRs for landscape or horticulture applications in subtropical regions.

分类号：

  • 相关文献

[1]Molecular cloning and expression analysis of tea plant aquaporin (AQP) gene family. Yue, Chuan,Cao, Hongli,Wang, Lu,Zhou, Yanhua,Hao, Xinyuan,Zeng, Jianming,Wang, Xinchao,Yang, Yajun,Wang, Lu,Zeng, Jianming,Wang, Xinchao,Yang, Yajun,Yue, Chuan,Cao, Hongli,Wang, Lu,Zhou, Yanhua,Hao, Xinyuan,Zeng, Jianming,Wang, Xinchao,Yang, Yajun.

[2]Differential expression of gibberellin- and abscisic acid-related genes implies their roles in the bud activity-dormancy transition of tea plants. Yue, Chuan,Cao, Hongli,Guo, Yuqiong,Ye, Naixing,Yue, Chuan,Cao, Hongli,Hao, Xinyuan,Zeng, Jianming,Qian, Wenjun,Yang, Yajun,Wang, Xinchao. 2018

[3]Comprehensive Transcriptome Analyses Reveal Differential Gene Expression Profiles of Camellia sinensis Axillary Buds at Para-, Endo-, Ecodormancy, and Bud Flush Stages. Hao, Xinyuan,Yang, Yajun,Yue, Chuan,Wang, Lu,Wang, Xinchao,Hao, Xinyuan,Yang, Yajun,Yue, Chuan,Wang, Lu,Wang, Xinchao,Horvath, David P.. 2017

[4]Flowering time regulation by the CONSTANS-Like gene OsCOL10. Tan, Junjie,Wu, Fuqing,Wan, Jianmin,Wan, Jianmin,Tan, Junjie. 2017

[5]Ectopic expression of soybean methionine synthase delays flowering time in transgenic tobacco plants. Gao, Z. L.,Wu, H.,Lin, D. Z.,Zhang, Q. L.,Chen, Y. H.,Gao, Z. L.,Wu, H.,Lin, D. Z.,Zhang, Q. L.,Chen, Y. H.,Sha, A. H.,Sha, A. H..

[6]Distribution of the photoperiod insensitive Ppd-D1a allele in Chinese wheat cultivars. Yang, F. P.,Zhang, X. K.,Xia, X. C.,He, Z. H.,Yang, F. P.,Yang, W. X.,Zhang, X. K.,Laurie, D. A.,He, Z. H..

[7]Genetic analysis and QTL detection of reproductive period and post-flowering photoperiod responses in soybean. Cheng, Lirui,Wang, Ying,Zhang, Chunbin,Wu, Cunxiang,Xu, Jianlong,Zhu, Huiying,Leng, Jiantian,Bai, Yangnian,Guan, Rongxia,Hou, Wensheng,Han, Tianfu,Cheng, Lirui,Zhang, Lijuan.

[8]OsCOL10, a CONSTANS-Like Gene, Functions as a Flowering Time Repressor Downstream of Ghd7 in Rice. Tan, Junjie,Jin, Mingna,Wu, Fuqing,Sheng, Peike,Cheng, Zhijun,Wang, Jiulin,Zheng, Xiaoming,Wang, Min,Zhu, Shanshan,Guo, Xiuping,Zhang, Xin,Wang, Haiyang,Wu, Chuanyin,Wan, Jianmin,Tan, Junjie,Sheng, Peike,Liu, Xuanming,Wan, Jianmin,Wang, Jiachang,Chen, Liping,Wang, Chunming,Wan, Jianmin,Tan, Junjie.

[9]The global adaptation of bread wheat at high latitudes. Trethowan, R. M.,Morgunov, Alexei,He, Zhonghu,De Pauw, R.,Crossa, J.,Warburton, M.,Baytasov, Arman,Zhang, Chunli,Mergoum, M.,Alvarado, G.. 2006

[10]Three FLOWERING LOCUS T-like genes function as potential florigens and mediate photoperiod response in sorghum. Wolabu, Tezera W.,Zhang, Fei,Niu, Lifang,Kalve, Shweta,Tadege, Million,Niu, Lifang,Bhatnagar-Mathur, Pooja,Muszynski, Michael G..

作者其他论文 更多>>

微信小程序