Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp avenae Strain RS-2

文献类型： 外文期刊

第一作者： Masum, Md. Mahidul Islam

作者： Masum, Md. Mahidul Islam;Yang, Yingzi;Li, Bin;Olaitan, Ogunyemi Solabomi;Chen, Jie;Zhang, Yang;Fang, Yushi;Qiu, Wen;Masum, Md. Mahidul Islam;Wang, Yanli;Sun, Guochang

作者机构：

关键词： Acidovorax avenae subsp;Avenae;T6SS;gene knock-out;pathogenicity;growth;biofilm;motility;Hcp

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2017 年 18 卷 10 期

页码：

收录情况： SCI

摘要： The Type VI secretion system (T6SS) is a class of macromolecular machine that is required for the virulence of gram-negative bacteria. However, it is still not clear what the role of T6SS in the virulence of rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) is. The aim of the current study was to investigate the contribution of T6SS in Aaa strain RS2 virulence using insertional deletion mutation and complementation approaches. This strain produced weak virulence but contains a complete T6SS gene cluster based on a genome-wide analysis. Here we compared the virulence-related phenotypes between the wild-type (RS-2) and 25 T6SS mutants, which were constructed using homologous recombination methods. The mutation of 15 T6SS genes significantly reduced bacterial virulence and the secretion of Hcp protein. Additionally, the complemented 7 mutations Delta pppA, Delta clpB, Delta hcp, Delta dotU, Delta icmF, Delta impJ, and Delta impM caused similar virulence characteristics as RS-2. Moreover, the mutant Delta pppA, Delta clpB, Delta icmF, Delta impJ and Delta impM genes caused by a 38.3 similar to 56.4% reduction in biofilm formation while the mutants Delta pppA, Delta clpB, Delta icmF and Delta hcp resulted in a 37.5 similar to 44.6% reduction in motility. All together, these results demonstrate that T6SS play vital roles in the virulence of strain RS-2, which may be partially attributed to the reductions in Hcp secretion, biofilm formation and motility. However, differences in virulence between strain RS-1 and RS-2 suggest that other factors may also be involved in the virulence of Aaa.

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